In vivo time-lapse imaging of zebrafish embryonic development.
In vivo time-lapse imaging of zebrafish embryonic development.
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DOI：
10.1101/pdb.prot4816
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发表时间：
2007-08-01
期刊：
影响因子：
--
通讯作者：
Koster, Reinhard W
中科院分区：
文献类型：
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作者：
Distel, Martin;Koster, Reinhard W
IntroductionIntravival延时成像是一种强大的技术，用于研究连续的发育过程，而不会错过关键事件。由于斑马鱼胚胎的快速胚胎发生、外部发育和透明性，它们的发育过程可以在活体的背景下在延时研究中可视化。以下方案描述了一种使用共聚焦或复合体视显微镜在几天内对斑马鱼胚胎进行活体延时成像的方法。
INTRODUCTIONIntravital time-lapse imaging is a powerful technique for investigating continuous developmental processes without missing crucial events. Because of the rapid embryogenesis, external development, and transparency of zebrafish embryos, their developmental processes can be visualized in time-lapse studies in the context of the living organism. The following protocol describes a method for performing intravital time-lapse imaging of zebrafish embryos over several days using confocal or compound stereomicroscopes.