Neurokinin B activates the formation and bone resorption activity of rat osteoclasts
Neurokinin B activates the formation and bone resorption activity of rat osteoclasts
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DOI：
10.1016/j.npep.2011.03.006
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发表时间：
2011-06-01
期刊：
影响因子：
2.9
通讯作者：
Goto, Tetsuya
中科院分区：
文献类型：
--
作者：
Ichiki, Takako;Kuroishi, Kayoko N.;Goto, Tetsuya
神经激肽B（NKB）是速激肽家族中的一种神经肽，主要在中枢神经系统中充当神经递质和神经调质。NKB及其受体神经激肽-3受体（NK-3R）在外周组织中的分布和作用知之甚少。本研究探讨了NKB和NK-3R在外周组织中的分布，以及NKB在骨代谢中的作用，特别是通过NK-3R在破骨细胞形成和骨吸收活性中的作用。用免疫组织化学方法观察NKB在大鼠三叉神经节（TG）神经元和牙周组织轴突中的分布。以体外培养的大鼠骨髓破骨细胞为材料，采用免疫细胞化学和RT-PCR方法检测NK-3R在破骨细胞中的分布，并观察NKB对破骨细胞在象牙片上吸收活性的影响。我们发现，NKB免疫阳性神经元定位在大鼠TG和NKB免疫阳性轴突分布在牙周组织。在培养的破骨细胞中发现NK-3R的免疫反应性，并通过RT-PCR证实了NK-3R mRNA在破骨细胞中的表达。与对照组相比，NKB的加入显著增加了破骨细胞的数量和吸收面积。这些结果表明，NKB定位于外周神经元，并可能涉及通过NK-3R激活破骨细胞的形成和骨吸收。(C)2011爱思唯尔有限公司保留所有权利。
Neurokinin B (NKB) is a neuropeptide in the tachykinin family that acts as a neurotransmitter and neuromodulator, primarily in the central nervous system. The distribution and role of NKB and its receptor, the neurokinin-3 receptor (NK-3R), in peripheral tissues are poorly understood. In this study, we investigated the distribution of NKB and NK-3R in peripheral tissues as well as the role of NKB in bone metabolism, especially in osteoclast formation and bone resorption activity through NK-3R. The distributions of NKB in intact rat neurons of the trigeminal ganglion (TG) and in axons of periodontal tissue were investigated by immunohistochemistry. Osteoclasts from cultured rat bone marrow cells were used to examine the distribution of NK-3R by immunocytochemistry and RT-PCR and to investigate the effects of NKB on the resorption activity of osteoclasts on ivory slices. We found that NKB immunopositive neurons were localized in the rat TG and that NKB immunopositive axons were distributed in periodontal tissues. Immunoreactivity for NK-3R was found in cultured osteoclasts, and NK-3R mRNA expression in the osteoclasts was confirmed by RT-PCR. The addition of NKB significantly increased the number of osteoclasts and the resorption area compared with the control. These findings suggest that NKB was localized in peripheral neurons and may involve the activation of osteoclast formation and bone resorption through NK-3R. (C) 2011 Elsevier Ltd. All rights reserved.