Speed of signaltransfer in the chloroplast accumulationresponse
Speed of signaltransfer in the chloroplast accumulationresponse
复制标题
叶绿体积累反应中信号传递的速度
DOI：
10.1007/s10265-009-0284-y
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
M. Wada
中科院分区：
文献类型：
--
作者：
Tsuboi;H.;M. Wada
叶绿体光迁移运动的光感受器是已知的，但是由光感受器产生的信号仍然是未知的。为了了解叶绿体积累运动信号的性质，我们测定了铁线蕨配子体中光照射区向叶绿体传递信号的速度。用不同光强的红光或蓝光微束照射暗适应配子体细胞1 min或连续照射，叶绿体开始向照射区移动。从开始移动的时间和叶绿体到微束的距离之间的关系计算信号传递的速度，并且发现在任何光条件下都是恒定的。在原叶体细胞中，向基部的移动速度约为1.0 μm min ~（-1），在原丝体细胞中，向基部的移动速度约为0.7 μm min ~（-1），向顶端的移动速度约为2.3 μm min ~（-1）。我们证实了在连续蓝光照射下拟南芥叶肉细胞的信号传递速度约为0.8 μm min-1。根据信号传输的速度讨论了信号的可能候选者。
The photoreceptors for chloroplast photorelocation movement have been known, but the signal(s) raised by photoreceptors remains unknown. To know the properties of the signal(s) for chloroplast accumulation movement, we examined the speed of signal transferred from light-irradiated area to chloroplasts in gametophytes ofAdiantum capillus-veneris. When dark-adapted gametophyte cells were irradiated with a microbeam of various light intensities of red or blue light for 1min or continuously, the chloroplasts started to move towards the irradiated area. The speed of signal transfer was calculated from the relationship between the timing of start moving and the distance of chloroplasts from the microbeam and was found to be constant at any light conditions. In prothallial cells, the speed was about 1.0 μm min-1 and in protonemal cells about 0.7 μm min-1 towards base and about 2.3 μm min-1 towards the apex. We confirmed the speed of signal transfer inArabidopsis thalianamesophyll cells under continuous irradiation of blue light, as was about 0.8 μm min-1. Possible candidates of the signal are discussed depending on the speed of signal transfer.