Rapid identification of consistent novel QTLs underlying long-juvenile trait in soybean by multiple genetic populations and genotyping-by-sequencing
Rapid identification of consistent novel QTLs underlying long-juvenile trait in soybean by multiple genetic populations and genotyping-by-sequencing
复制标题
通过多个遗传群体和测序基因分型快速鉴定大豆长幼年性状的一致新QTL
DOI：
10.1007/s11032-019-0979-2
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发表时间：
2019-06-01
影响因子：
3.1
通讯作者：
Lu, Sijia
中科院分区：
文献类型：
--
作者：
Fang, Chao;Chen, Liyu;Lu, Sijia
开花时间是控制作物产量和纬度适应性的重要因素。大豆是兼性短日照（SD）植物，来自温带地区的栽培品种在低纬度地区生长时通常经历非常短的幼年期和早期开花，从而导致极低的谷物产量。然而，引入长幼龄（LJ）性状，延长营养阶段，提高产量在SD环境下，在很大程度上克服了这一限制，使大豆生产扩大到热带地区。数量性状基因座（QTL）定位是鉴定与LJ性状相关的新基因座的基本方法，而传统的方法需要花费大量的时间来验证结果。在这里，我们提供了一个新的策略，快速确定一致的新的QTL，通过使用多个遗传群体和基因分型测序（GBS）的方法。在本研究中，我们开发了六个F2群体，并在SD条件下生长。通过对开花时间（R1）和高密度遗传图谱的整合，共定位到7个与LJ性状相关的QTL。在不同群体中重复检测到3个一致的QTL，其中1个为已有报道，2个为新发现。本研究不仅为LJ性状候选基因的精细定位提供了基础资源，也为QTL定位提供了新的思路。
Flowering time is an important factor that controls crop yield and latitudinal adaptation. Soybean is a facultative short-day (SD) plant, and cultivars that originate from temperate regions generally undergo a very short juvenile phase and early flowering when grown in low-latitude regions, thereby resulting in extremely low grain yield. However, introduction of the long-juvenile (LJ) trait, which extends the vegetative phase and improves yield under SD environments, has largely overcome this constraint and has enabled the expansion of soybean production to tropical regions. Quantitative trait locus (QTL) mapping is a fundamental approach to the identification of new loci related to LJ trait, while conventional strategy takes lots of time to verify the findings. Here, we provide a new strategy to rapidly identify consistent novel QTLs by using multiple genetic populations and genotyping-by-sequencing (GBS) approach. In this study, we developed six F2populations and grew them under SD conditions. A total of seven QTLs related to the LJ trait were identified by integrating flowering time (R1) and high-density genetic maps. There were three consistent QTLs repeatedly detected in different populations and one of them has been previously reported, yet two are novel. This research not only provides fundamental resources for fine-mapping candidate genes associated to the LJ trait, but also provides new insights to QTL mapping.