Designing tailored biomaterial surfaces to direct keratinocyte morphology, attachment, and differentiation.
Designing tailored biomaterial surfaces to direct keratinocyte morphology, attachment, and differentiation.
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设计定制的生物材料表面以指导角质形成细胞的形态、附着和分化。
DOI：
10.1002/jbm.a.32168
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Pins,GD
中科院分区：
文献类型：
--
作者：
Bush,KA;Driscoll,PF;Soto,ER;Lambert,CR;McGimpsey,WG;Pins,GD
精确地设计生物材料的表面化学，以调节指导细胞功能的生化信号分子的吸附和功能，对于组织工程支架的发展至关重要。具体来说，本研究描述了使用功能化自组装单层（SAMs）作为模型系统来评估生物材料表面特性对控制纤维连接蛋白（FN）构象和浓度以及角化细胞功能的影响。通过系统分析低表面密度和饱和表面密度下的FN吸附，我们区分了SAM依赖的FN浓度和构象对呈现指导细胞功能的细胞结合域的影响。免疫染色样品的定量图像分析显示，通过整合素介导的信号机制，调节FN协同位点的可用性与角化细胞附着、扩散和分化的变化直接相关。这项研究的结果将用于阐明可纳入皮肤等效物和经皮植入物的设计特征，以提高再上皮化和组织再生的速度。此外，这些发现表明，基于SAM的模型系统是设计和研究支架开发的有价值的工具，这些支架可以调节细胞外基质线索的构象和加速组织再生速率的细胞功能。©2008 Wiley期刊公司[J]生物医学工程学报，2009
Precisely engineering the surface chemistry of biomaterials to modulate the adsorption and functionality of biochemical signaling molecules that direct cellular functions is critical in the development of tissue engineered scaffolds. Specifically, this study describes the use of functionalized self‐assembled monolayers (SAMs) as a model system to assess the effects of biomaterial surface properties on controlling fibronectin (FN) conformation and concentration as well as keratinocyte function. By systematically analyzing FN adsorption at low and saturated surface densities, we distinguished between SAM‐dependent effects of FN concentration and conformation on presenting cellular binding domains that direct cellular functions. Quantitative image analyses of immunostained samples showed that modulating the availability of the FN synergy site directly correlated with changes in keratinocyte attachment, spreading, and differentiation, through integrin‐mediated signaling mechanisms. The results of this study will be used to elucidate design features that can be incorporated into dermal equivalents and percutaneous implants to enhance the rate of re‐epithelialization and tissue regeneration. Furthermore, these findings indicate that SAM‐based model systems are a valuable tool for designing and investigating the development of scaffolds that regulate the conformation of extracellular matrix cues and cellular functions that accelerate the rate of tissue regeneration. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009