EXPRESSION OF DROSOPHILA HEAT-SHOCK COGNATE GENES DURING HEAT-SHOCK AND DEVELOPMENT
EXPRESSION OF DROSOPHILA HEAT-SHOCK COGNATE GENES DURING HEAT-SHOCK AND DEVELOPMENT
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DOI：
10.1016/0012-1606(83)90291-9
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发表时间：
1983-01-01
影响因子：
2.7
通讯作者：
MANSEAU, LJ
中科院分区：
文献类型：
--
作者：
CRAIG, EA;INGOLIA, TD;MANSEAU, LJ
果蝇基因组包含许多与果蝇的主要热休克诱导（Hsp70）基因相关的基因。这些热休克同源（Hsc）基因的结构和表达进行了研究; Hsc 1（如前所述），Hsc 2和Hsc 4分散在3号染色体上的细胞学位点70 C，87 D和88 E，分别。通过杂交选择翻译实验显示与Hsc 4同源的RNA编码apprx蛋白质。70kd [千道尔顿]，具有与热休克诱导的70K蛋白相似但可区分的迁移率。由于5 "非蛋白质编码区大小的差异，有可能在成年果蝇中识别出每个基因的不同转录本。与Hsp基因的转录本不同，热休克后Hsc转录本的丰度没有增加。这些Hsc基因的转录在发育过程中受到调节。虽然从Hsc4转录同样丰富的胚胎，幼虫和成人分离的RNA，Hsc1和Hsc2转录没有检测到胚胎和幼虫的RNA，因此在这些阶段中的丰富程度至少比成人少20倍。比较了与编码Hsp70氨基末端同源的区域的一级DNA序列。这4个基因的碱基序列同源性为76%，推导的氨基酸序列同源性为78%。Hsp70基因不含插入序列; Hsc4在编码前101个氨基酸的区域中不含相对于Hsp70的插入。在指定aa66的密码子中，Hsc 1被1.7kb的DNA [DNA酶]中断;在aa55的密码子中，Hsc 2含有插入的. apprx。650个B。在Hsc 1和Hsc 2中插入的5 "和3"连接处的序列与在果蝇和许多其他生物体的插入序列中发现的序列相似。
The Drosophila genome contains a number of genes related to the major heat-shock-inducible (Hsp70) genes of Drosophila. The structure and expression of 3 of these heat-shock cognate (Hsc) genes were studied; Hsc1 (previously described), Hsc2 and Hsc4 are dispersed on chromosome 3 at cytological loci 70C, 87D and 88E, respectively. RNA homologous to Hsc4 was shown by hybridization selection translation experiments to encode a protein of .apprx. 70kd [kilodalton], with similar, but distinguishable, mobility to that of the heat-shock-inducible 70K protein. Because of variance in size of the 5'' nonprotein coding region, it was possible to identify in adult flies distinct transcripts from each gene. Unlike transcripts from Hsp genes, the abundance of the Hsc transcripts was not increased after heat shock. Transcription of these Hsc genes is regulated during development. While transcripts from Hsc4 were equally abundant in RNA isolated from embryos, larvae and adults, Hsc1 and Hsc2 transcripts were not detected in embryo and larval RNA, and are therefore at least 20 times less abundant in these stages than in adults. The primary DNA sequences of the regions homologous to that encoding the amino terminus of Hsp70 were compared. Approximately 76% homology of the base sequence and 78% homology of the deduced amino acid sequence were found among the 4 genes. The Hsp70 genes contain no intervening sequences; Hsc4 contains no insertions relative to Hsp70 in the region encoding the first 101 aa. In the codon specifying aa66 Hsc1 is interrupted by 1.7 kb [kilobase] of DNA; in the codon for aa55 Hsc2 contains an insertion of .apprx. 650 b. The sequences at the 5'' and 3'' junctions of the insertions in both Hsc1 and Hsc2 are similar to those found in intervening sequences of Drosophila and of many other organisms.