Involvement of actin and myosins in Plasmodium berghei ookinete motility
Involvement of actin and myosins in Plasmodium berghei ookinete motility
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DOI：
10.1016/j.molbiopara.2006.09.003
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发表时间：
2006-12-01
影响因子：
1.5
通讯作者：
Louis, Christos
中科院分区：
文献类型：
--
作者：
Siden-Kiamos, Inga;Pinder, Jennifer C.;Louis, Christos
疟原虫属的动植物是一种活动的、侵袭性的细胞，在蚊子中肠中摄取了受寄生虫感染的血粉后，这些细胞就会发育。我们在这里展示了在昆虫细胞存在的情况下，动子在玻片上表现出滑动运动。此外，除了细胞的静止“弯曲”和“旋转”之外，还发生了两种不同类型的运动：平均速度约为6微米/分钟的直段的生产性向前移位运动和不导致向前移位的旋转运动。肌球蛋白ATPase的抑制剂丁二酮单肟和三种不同的肌动蛋白抑制剂可以减少运动。我们还研究了编码肌动蛋白和两个小类XIV肌球蛋白PbMyoA和PbMyoB的基因在卵子发育过程中的表达。免疫印迹显示，PbMyoA只存在于完全成熟的卵子中，而另外两种蛋白在配子体和受精卵中也有表达。免疫荧光实验表明，MyoA和肌动蛋白共同定位于寄生虫的顶端，而MYOB在整个细胞周围呈点状表达。在用jasplakinolide处理后，可检测到的肌动蛋白的表观水平似乎显著增加，并集中在卵母细胞基极的一个离散区域。(C)2006爱思唯尔B.V.保留所有权利。
Ookinetes of the genus Plasmodium are motile, invasive cells that develop in the mosquito midgut following ingestion of a parasite- infected blood meal. We show here that ookinetes display gliding motility on glass slides in the presence of insect cells. Moreover, in addition to stationary "flexing" and "twirling" of the cells, two distinct types of movements occur: productive forward translocational motility in straight segment that progresses with an average speed of approximately 6 mu m/min and rotational motility, which does not lead to forward translocation. Locomotion is reduced by treatment with butanedione monoxime, an inhibitor of myosin ATPase, and by three different actin inhibitors. We also studied the expression during ookinete development of genes encoding actin and two small class XIV myosins, PbMyoA, and PbMyoB. Western immunoblots revealed that PbMyoA is only present in fully mature ookinetes, whilst the other two proteins are additionally expressed in gametocytes and zygotes. Immunofluorescence experiments reveal that MyoA and actin co-localize in the apical tip of the parasite whereas MyoB displays a punctate pattern of expression around the entire cell periphery. Following treatment with jasplakinolide, the apparent level of detectable actin appears to substantially increase and becomes concentrated in a discrete area in the basal pole of the ookinete. (c) 2006 Elsevier B.V. All rights reserved.