Contamination of retail foods, particularly turkey from community markets (Minnesota, 1999-2000) with antimicrobial-resistant and extraintestinal pathogenic Escherichia coli
Contamination of retail foods, particularly turkey from community markets (Minnesota, 1999-2000) with antimicrobial-resistant and extraintestinal pathogenic Escherichia coli
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DOI：
10.1089/fpd.2005.2.38
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发表时间：
2005-03-01
影响因子：
2.8
通讯作者：
Tatini, Sita
中科院分区：
文献类型：
--
作者：
Johnson, James R.;Delavari, Parissa;Tatini, Sita
为了评估食品供应作为抗菌素耐药性和肠外致病性大肠杆菌 (ExPEC) 的可能载体，我们定义了不同零售食品中大肠杆菌的流行率、密度、克隆多样性、毒力特征和抗菌素耐药性概况。对从明尼阿波利斯-圣路易斯地区 16 个零售市场购买的 346 种食品（蔬菜、农产品、牛肉、猪肉、鸡肉和火鸡）进行了微生物学调查。 1999-2000 年在圣保罗地区进行了大肠杆菌的选择性培养，并对大肠杆菌分离株进行了广泛的分子和表型表征。肉类，特别是火鸡产品，经常受到具有抗药性的大肠杆菌和 ExPEC 的广泛污染，污染程度比农产品（甚至来自农贸市场的农产品）还要严重。此外，肉类来源的大肠杆菌在系统发育背景（更常见的是毒力相关的系统发育群体 B2 或 D）、毒力基因型（更广泛）和抗菌素耐药性特征（更广泛）方面与农产品来源大肠杆菌有很大不同。分子分型方法将四种火鸡源分离株与代表 ExPEC 克隆组 O7:K1:H-、O83:K1 和 O73/O77:K52:H18（“克隆组 A”）的选定人类临床和粪便分离株进行匹配。在杂货店购买的肉类，尤其是火鸡产品，经常受到耐药性大肠杆菌和 ExPEC 的污染。需要对这些食源性生物的起源和健康后果进行进一步研究，以澄清适当的监管和监测系统以及预防性干预措施的必要性并指导其可能的发展。
To assess the food supply as a possible vehicle for antimicrobial-resistant and extraintestinal pathogenic Escherichia coli (ExPEC), we defined the prevalence, density, clonal diversity, virulence characteristics, and antimicrobial resistance profiles of E. coli among diverse retail food items. A microbiological survey was undertaken of 346 food items (vegetables, produce, beef, pork, chicken, and turkey) purchased as a convenience sample from 16 retail markets within the Minneapolis-St. Paul area in 1999-2000, with selective cultures for E. coli and extensive molecular and phenotypic characterization of E. coli isolates. Meats, particularly turkey products, were often extensively contaminated with antimicrobial-resistant E. coli and ExPEC, to a much greater extent than were produce items, even those from farmer's markets. Moreover, meat-source E. coli differed substantially from produce-source E. coli with respect to phylogenetic background (more commonly from virulence-associated phylogenetic groups B2 or D), virulence genotype (more extensive), and antimicrobial resistance profile (more extensive). Molecular typing methods matched four turkey-source isolates to selected human clinical and fecal isolates representing the O7:K1:H-, O83:K1, and O73/O77:K52:H18 ("clonal group A") clonal groups of ExPEC. Meats purchased in grocery stores, particularly turkey products, are frequently contaminated with antimicrobial-resistant E. coli and ExPEC. Further study is needed regarding the origins and health consequences of these foodborne organisms, both to clarify the need for and to guide the possible development of appropriate regulatory and monitoring systems and preventive interventions.