BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs
BUSCO: assessing genome assembly and annotation completeness with single-copy orthologs
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DOI：
10.1093/bioinformatics/btv351
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发表时间：
2015-10-01
期刊：
影响因子：
5.8
通讯作者：
Zdobnov, Evgeny M.
中科院分区：
文献类型：
--
作者：
Simao, Felipe A.;Waterhouse, Robert M.;Zdobnov, Evgeny M.
动机：基因组学为生物学研究带来了革命性的变化，但组装序列的质量评估是复杂的，并且大多局限于N50等技术指标。结果：基于进化信息对基因内容的期望，提出了一种定量评估基因组组装和注释完整性的方法。我们在开源软件中实施了评估程序，并使用了一套名为BUSCO的基准通用单拷贝同源序列。
Motivation: Genomics has revolutionized biological research, but quality assessment of the resulting assembled sequences is complicated and remains mostly limited to technical measures like N50.Results: We propose a measure for quantitative assessment of genome assembly and annotation completeness based on evolutionarily informed expectations of gene content. We implemented the assessment procedure in open-source software, with sets of Benchmarking Universal Single-Copy Orthologs, named BUSCO.