CPF impedes cell cycle re-entry of quiescent lung cancer cells through transcriptional suppression of FACT and c-MYC
CPF impedes cell cycle re-entry of quiescent lung cancer cells through transcriptional suppression of FACT and c-MYC
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CPF 通过 FACT 和 c-MYC 转录抑制阻碍静止肺癌细胞的细胞周期重新进入
DOI：
10.1111/jcmm.14897
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发表时间：
2020-01-20
影响因子：
5.3
通讯作者：
Dong, Qihan
中科院分区：
文献类型：
--
作者：
Bi, Ling;Xie, Chanlu;Dong, Qihan
阻断静止期癌细胞的细胞周期重新进入是防止癌症进展和复发的一种策略。我们研究了黄连、半夏和天花粉合剂(黄连、半夏和天花粉)在阻止静止的肺癌细胞增殖转换方面的作用和作用方式。结果表明，CPF通过降低FACT和c-MYC的mRNA和蛋白水平，使FACT和c-MYC启动子区域的H3K4三甲基化和RNA聚合酶II的占有率降低，从而阻碍了静止期肺癌细胞的细胞周期重入。将暴露在CPF中的静止癌细胞植入动物体内后，肿瘤体积/重量都有所减少。因此，CPF通过转录抑制FACT和c-myc来抑制增殖转换，为预防肿瘤复发的治疗靶点和干预方法提供了新的视角。
Blockade of cell cycle re-entry in quiescent cancer cells is a strategy to prevent cancer progression and recurrence. We investigated the action and mode of action of CPF mixture (Coptis chinensis, Pinellia ternata and Fructus trichosanthis) in impeding a proliferative switch in quiescent lung cancer cells. The results indicated that CPF impeded cell cycle re-entry in quiescent lung cancer cells by reduction of FACT and c-MYC mRNA and protein levels, with concomitant decrease in H3K4 tri-methylation and RNA polymerase II occupancy at FACT and c-MYC promoter regions. Animals implanted with quiescent cancer cells that had been exposed to CPF had reduced tumour volume/weight. Thus, CPF suppresses proliferative switching through transcriptional suppression of FACT and the c-MYC, providing a new insight into therapeutic target and intervention method in impeding cancer recurrence.