Genome-wide identification and analysis of the EIN3/EIL gene family in allotetraploid Brassica napus reveal its potential advantages during polyploidization
Genome-wide identification and analysis of the EIN3/EIL gene family in allotetraploid Brassica napus reveal its potential advantages during polyploidization
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异源四倍体甘蓝型油菜 EIN3/EIL 基因家族的全基因组鉴定和分析揭示了其在多倍化过程中的潜在优势
DOI：
10.1186/s12870-019-1716-z
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发表时间：
2019-03-21
影响因子：
5.3
通讯作者：
Wang, Jianbo
中科院分区：
文献类型：
--
作者：
Li, Mengdi;Wang, Ruihua;Wang, Jianbo
背景资料：多倍化是被子植物进化史上的一个普遍事件，植物多倍化后除了简单的基因组加倍外，还会发生基因组的一些变化。异源四倍体甘蓝型油菜（Brassica napus）及其二倍体祖先（B. rapa和B. oleracea）是研究与多倍化相关的问题的良好群体。另一方面，EIN 3/EIL基因家族是植物中一个重要的基因家族，其所有成员都是乙烯信号通路中的关键基因。到目前为止，B.结果：在B中共鉴定出13个、7个和7个EIN 3/EIL基因。napus（2n = 4x = 38，A（n）C（n）），B. rapa（2n = 2x = 20，A（r））和B. oleracea（2n = 2x = 18，C-o）。EIN 3/EIL蛋白可分为3个进化枝，并进一步分为8个亚进化枝。Ka/Ks分析表明，所有EIN 3/EIL基因在重复后都经过了纯化选择。此外，基因结构分析表明，B.同源基因表达偏性分析表明，B. napus偏向于其二倍体祖先B。拉帕。B.与二倍体亲本相比，油菜含有更多的顺式作用元件，这些元件主要与胚乳基因表达和光响应有关。因此，B。结论：甘蓝型油菜为异源四倍体B。油菜在某些生物学方面可能具有潜在的优势。此外，我们的研究结果可以增加对B中EIN 3/EIL基因家族进化的理解。为今后多倍体化的研究提供参考。
Background: Polyploidization is a common event in the evolutionary history of angiosperms, and there will be some changes in the genomes of plants other than a simple genomic doubling after polyploidization. Allotetraploid Brassica napus and its diploid progenitors (B. rapa and B. oleracea) are a good group for studying the problems associated with polyploidization. On the other hand, the EIN3/EIL gene family is an important gene family in plants, all members of which are key genes in the ethylene signaling pathway. Until now, the EIN3/EIL gene family in B. napus and its diploid progenitors have been largely unknown, so it is necessary to comprehensively identify and analyze this gene family.Results: In this study, 13, 7 and 7 EIN3/EIL genes were identified in B. napus (2n = 4x = 38, A(n)C(n)), B. rapa (2n = 2x = 20, A(r)) and B. oleracea (2n = 2x = 18, C-o). All of the identified EIN3/EIL proteins were divided into 3 clades and further divided into 8 sub-clades. Ka/Ks analysis showed that all identified EIN3/EIL genes underwent purifying selection after the duplication events. Moreover, gene structure analysis showed that some EIN3/EIL genes in B. napus acquired introns during polyploidization, and homolog expression bias analysis showed that B. napus was biased towards its diploid progenitor B. rapa. The promoters of the EIN3/EIL genes in B. napus contained more cis-acting elements, which were mainly involved in endosperm gene expression and light responsiveness, than its diploid progenitors. Thus, B. napus might have potential advantages in some biological aspects.Conclusions: The results indicated allotetraploid B. napus might have potential advantages in some biological aspects. Moreover, our results can increase the understanding of the evolution of the EIN3/EIL gene family in B. napus, and provided more reference for future research about polyploidization.