PHYSICOCHEMICAL CHARACTERIZATION OF LARGE UNILAMELLAR PHOSPHOLIPID-VESICLES PREPARED BY REVERSE-PHASE EVAPORATION
PHYSICOCHEMICAL CHARACTERIZATION OF LARGE UNILAMELLAR PHOSPHOLIPID-VESICLES PREPARED BY REVERSE-PHASE EVAPORATION
复制标题
DOI：
10.1016/0005-2736(83)90214-6
复制
发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
PAPAHADJOPOULOS, D
中科院分区：
文献类型：
--
作者：
DUZGUNES, N;WILSCHUT, J;PAPAHADJOPOULOS, D
研究了由磷脂酰胆碱组成的大单层囊泡(LUV)的性质，并与小单层囊泡(SUV)和多层囊泡(MLV)进行了比较。以Pr3+为位移试剂的~1H-核磁共振研究表明了LUV的单层性质。用差示扫描量热法研究了由二棕榈酰磷脂酰胆碱(DPPC)组成的LUV的凝胶-液晶相变、二苯基己三烯的荧光偏振度和90度。光散射，发生在略低的温度(40.8度)。C)高于MLV(42℃)。C)，并通过.apprx进行扩展。50%。SUV的相变被转移到相当低的温度(中点，38度)。C)并且扩展到很宽的温度范围。在LUV中，没有观察到明确的预跃迁。由羧基荧光素泄漏监测的LUV(DPPC)的渗透性在相变温度下急剧增加，且释放程度大于MLV。SUV在很宽的温度范围内都会发生泄漏。LUV(DPPC)的冷冻断口电子显微镜显示直径0.1-0.2µm的小泡，断裂面大多光滑。在低于相变的温度下，群体中较大的囊泡具有成角的表面，挤出的MLV也是如此。在MLV中，在过渡前和主过渡之间的温度下可以看到带状图案，在较小的LUV中没有观察到，尽管较大的小泡显示出酒窝的外观。
Properties of large unilamellar vesicles (LUV), composed of phosphatidylcholine and prepared by reverse-phase evaporation and subsequent extrusion through Unipore polycarbonate membranes, were investigated and compared with those of small unilamellar vesicles (SUV) and of multilamellar vesicles (MLV). The unilamellar nature of the LUV is shown by 1H-NMR using Pr3+ as a shift reagent. The gel to liquid-crystalline phase transition of LUV composed of dipalmitoylphosphatidylcholine (DPPC) monitored by differential scanning calorimetry, fluorescence polarization of diphenylhexatriene and 90.degree. light scattering, occurs at a slightly lower temperature (40.8.degree. C) than that of MLV (42.degree. C) and is broadened by .apprx. 50%. The phase transition of SUV is shifted to considerably lower temperatures (mid-point, 38.degree. C) and extends over a wide temperature range. In LUV a well-defined pretransition is not observed. The permeability of LUV (DPPC) monitored by leakage of carboxyflourescein, increases sharply at the phase transition temperature, and the extent of release is greater than that from MLV. Leakage from SUV occurs in a wide temperature range. Freeze-fracture EM of LUV (DPPC) reveals vesicles of 0.1-0.2 .mu.m diameter with mostly smooth fracture faces. At temperatures below the phase transition, the larger vesicles in the population have angled faces, as do extruded MLV. A banded pattern, seen in MLV at temperatures between the pretransition and the main transition, is not observed in the smaller LUV, although the larger vesicles reveal a dimpled appearance.