RESTRICTION FRAGMENT LENGTH POLYMORPHISM LINKAGE MAP FOR ARABIDOPSIS-THALIANA
RESTRICTION FRAGMENT LENGTH POLYMORPHISM LINKAGE MAP FOR ARABIDOPSIS-THALIANA
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DOI：
10.1073/pnas.85.18.6856
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发表时间：
1988-09-01
影响因子：
11.1
通讯作者：
MEYEROWITZ, EM
中科院分区：
文献类型：
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作者：
CHANG, C;BOWMAN, JL;MEYEROWITZ, EM
我们构建了开花植物拟南芥核基因组的限制性片段长度多态性连锁图谱。该图谱包含90个随机分布的分子标记，物理上非常密集; > 50%的基因组在1.9厘摩或约1.9厘摩内。270个内切酶对，映射的DNA片段。该图谱是基于两个不同杂交组合中标记的减数分裂分离。限制性片段长度多态性连锁群与五个经典映射的连锁群，凭借映射的突变，包括在这些交叉整合。标记物由拟南芥基因克隆和随机低拷贝数基因组DNA克隆组成，其能够用限制性酶EcoRI、Bgl II和/或Xba I检测多态性。这些克隆的标记可以作为染色体步移的起点，允许分离已知图谱位置的Arbidiopsis基因。限制性片段长度多态性图谱还可以将未知基因功能的克隆与突变表型相关联，反之亦然。
We have constructed a restriction fragment length polymorphism linkage map for the nuclear genome of the flowering plant Arabidopsis thaliana. The map, containing 90 randomly distributed molecular markers, is physically very dense; > 50% of the genome is within 1.9 centimorgans, or .apprxeq. 270 kilobase pairs, of the mapped DNA fragments. The map was based on the meiotic segregation of markers in two different crosses. The restriction fragment length polymorphism linkage groups were integrated with the five classically mapped linkage groups by virtue of mapped mutations included in these crosses. Markers consist of both clones Arabidopsis geness and random low-copy-number genomic DNA clones that are able to detect polymorphisms with the restriction enzymes EcoRI, Bgl II, and/or Xba I. These cloned markers can serve as starting points for chromosome walking, allowing for the isolation of Arbidiopsis genes of known map location. The restriction fragment length polymorphism map also can associate clones of unknown gene function with mutant phenotypes, and vice versa.