One-pot facile synthesis of PEGylated superparamagnetic iron oxide nanoparticles for MRI contrast enhancement
One-pot facile synthesis of PEGylated superparamagnetic iron oxide nanoparticles for MRI contrast enhancement
复制标题
一锅法轻松合成聚乙二醇化超顺磁性氧化铁纳米颗粒用于增强 MRI 对比度
DOI：
10.1016/j.msec.2014.04.041
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发表时间：
2014-08-01
影响因子：
7.9
通讯作者：
Zhang, Bingbo
中科院分区：
文献类型：
--
作者：
Dai, Lingling;Liu, Yongkang;Zhang, Bingbo
采用一锅法制备了聚乙二醇（PEG）包覆的超顺磁性氧化铁纳米粒子（PEG.SPIONs）。合成的PEG. SPION大小均匀，平均流体动力学直径为11.7 nm。PEG. SPION在水中表现出优异的溶解性、胶体稳定性和生物相容性。磁共振成像（MRI）性能的PEG. SPION的特点是在体外和体内。在T1和T2加权成像中，双对比度均得到良好增强，纵向和横向弛豫率（r（1），r（2））分别为35.92 s（-1）/mM Fe 3+和206.91 s（-1）/mM Fe 3+。在体内的T-2加权MRI显示肝脏和脾脏明显增强，但在T-1加权MRI中没有。纳米颗粒的积聚主要在肝脏、脾脏和肠道中发现，而在肾脏、心脏和肺中的摄取要低得多。在14天内通过肝胆（HB）处理观察到PEG. SPION的逐渐排泄。还在体外和体内评价了PEG. SPION的毒性。通过苏木精-伊红染色后研究器官组织，发现SPION具有生物相容性。研究结论表明，PEG. SPION在医学MRI中具有很高的潜力。(C)2014爱思唯尔有限公司版权所有。
Polyethylene glycol (PEG)-coated superparamagnetic iron oxide nanoparticles (PEG.SPIONs) were prepared by a facile one-pot approach. The synthesized PEG.SPIONs were found to be uniform in size with an average hydrodynamic diameter of 11.7 nm. PEG.SPIONs exhibited excellent dispersibility in water, colloidal stability, and biocompatibility. The magnetic resonance imaging (MRI) properties of PEG.SPIONs were characterized both in vitro and in vivo. The dual contrast both in T-1 and T-2-weighted imaging was well enhanced with longitudinal and transverse relaxivity (r(1), r(2)) of 35.92 s(-1) per mM of Fe3+ and 206.91 s(-1) per mM of Fe3+ respectively. In vivo T-2-weighted MRI shows pronounced enhancement in the liver and spleen but not in T-1-weighted MRI. Accumulations of nanoparticles were found primarily in the liver, spleen, and intestine, while much lower uptake in the kidney, heart, and lungs. A gradual excretion of PEG.SPIONs was observed via hepatobiliary (HB) processing over a period of 14 days. The toxicity of PEG.SPIONs was also evaluated in vitro and in vivo. PEG.SPIONs were found to be biocompatible by investigating organ tissues after hematoxylin-eosin staining. The conclusion of the study indicates a high potential of PEG.SPIONs in medical MRI. (C) 2014 Elsevier B.V. All rights reserved.