Molecular Analysis of the Wheat Blast Population in Brazil with a Homolog of Retrotransposon MGR583
Molecular Analysis of the Wheat Blast Population in Brazil with a Homolog of Retrotransposon MGR583
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用逆转录转座子 MGR583 的同源物对巴西麦瘟群体进行分子分析
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
S. Mayama
中科院分区：
文献类型：
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作者：
A. S. Urashima;Y. Hashimoto;L. D. Don;M. Kusaba;Y. Tosa;H. Nakayashiki;S. Mayama
我们在从指粟 (Eleusine coracana) 中分离出来的稻瘟病菌 (Magnaporthe grisea) 的基因组中鉴定出了一系列分散的重复 DNA 序列。 Southern 印迹分析表明，该元件以中等拷贝数（30-40 个拷贝）存在于小麦（Triticum aestivum）稻瘟病分离物的基因组中。 DNA序列数据表明该元件包含与MGR583（一种多A型逆转录转座子）的逆转录酶结构域高度同源的区域。使用该元件的逆转录酶结构域作为分子探针，检查了小麦瘟病群体的遗传结构。 DNA指纹分析显示，感染小麦的分离株构成了它们自己的一个独立的、单一的谱系，这表明它们来自单一的起源。
We have identified a family of dispersed repetitive DNA sequences in the genome of a Magnaporthe grisea isolate from finger millet (Eleusine coracana). Southern blot analyses showed that this element is present in a moderate copy number (30-40 copies) in the genome of blast isolates from wheat (Triticum aestivum). DNA sequence data suggested that this element contains a region highly homologous to the reverse transcriptase domain of MGR583, a poly A-type retrotransposon. Using the reverse transcriptase domain of this element as a molecular probe, the genetic structure of the wheat blast population was examined. DNA fingerprinting analyses revealed that the wheat-infecting isolates constitute a separate, single lineage of their own, suggesting that they are derived from a single origin.