Comparative Transcriptome Analysis of the Pacific Oyster Crassostrea gigas Characterized by Shell Colors: Identification of Genetic Bases Potentially Involved in Pigmentation.
Comparative Transcriptome Analysis of the Pacific Oyster Crassostrea gigas Characterized by Shell Colors: Identification of Genetic Bases Potentially Involved in Pigmentation.
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以贝壳颜色为特征的太平洋牡蛎的比较转录组分析：鉴定可能参与色素沉着的遗传碱基
DOI：
10.1371/journal.pone.0145257
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Kong L
中科院分区：
文献类型：
--
作者：
Feng D;Li Q;Yu H;Zhao X;Kong L
研究背景贝类壳色多态性对贝类进化生物学和群体遗传学的发展具有重要意义，但其遗传基础和分子机制尚不清楚。太平洋牡蛎（Crassostrea gigas）是世界上最重要的养殖牡蛎之一。通过家系选择，获得了白色、金黄色、黑色和部分着色的四种壳色变异体。gigas已经开发出来了。为阐明牡蛎壳着色的遗传机制，gigas和促进具有所需着色模式的优良牡蛎品系的选择，通过RNA-seq在四种壳色变体中鉴定差异表达基因（DEG）。每个样本的数字基因表达产生了超过1500万个读数，产生了28，027个基因的表达数据。经配对比较，共鉴定出2,645个DEG，其中在白色、黑色、金色和部分着色壳中特异性表达上调的基因分别为432、91、43和39个。gigas，分别。ABC转运蛋白超家族中的Abca 1、Abca 3和Abcb 1基因与白色壳的形成显著相关。酪氨酸酶转录物（CGI_10008737）呈现一致的上调模式，呈金色。我们提出C.长吻鳕可以通过“内吞作用”下调Notch水平，防止壳色素沉着。结论本研究通过RNA-seq技术发现了一些潜在的壳色基因及其相关分子机制，为进一步研究壳色基因提供了基础信息，并有助于壳色基因的选育。千兆。
Background Shell color polymorphisms of Mollusca have contributed to development of evolutionary biology and population genetics, while the genetic bases and molecular mechanisms underlying shell pigmentation are poorly understood. The Pacific oyster (Crassostrea gigas) is one of the most important farmed oysters worldwide. Through successive family selection, four shell color variants (white, golden, black and partially pigmented) of C. gigas have been developed. To elucidate the genetic mechanisms of shell coloration in C. gigas and facilitate the selection of elite oyster lines with desired coloration patterns, differentially expressed genes (DEGs) were identified among the four shell color variants by RNA-seq. Results Digital gene expression generated over fifteen million reads per sample, producing expression data for 28,027 genes. A total number of 2,645 DEGs were identified from pair-wise comparisons, of which 432, 91, 43 and 39 genes specially were up-regulated in white, black, golden and partially pigmented shell of C. gigas, respectively. Three genes of Abca1, Abca3 and Abcb1 which belong to the ATP-binding cassette (ABC) transporters super-families were significantly associated with white shell formation. A tyrosinase transcript (CGI_10008737) represented consistent up-regulated pattern with golden coloration. We proposed that white shell variant of C. gigas could employ “endocytosis” to down-regulate notch level and to prevent shell pigmentation. Conclusion This study discovered some potential shell coloration genes and related molecular mechanisms by the RNA-seq, which would provide foundational information to further study on shell coloration and assist in selective breeding in C. gigas.