Low impact to fixed cell processing aiming transmission electron microscopy
Low impact to fixed cell processing aiming transmission electron microscopy
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DOI：
10.1590/0074-02760150433
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发表时间：
2016-06-01
期刊：
影响因子：
--
通讯作者：
Barreto-Vieira, Debora Ferreira
中科院分区：
文献类型：
--
作者：
Barth, Ortrud Monika;Silva, Marcos Alexandre Nunes da;Barreto-Vieira, Debora Ferreira
在细胞培养中，细胞结构在用于电子显微镜观察的处理过程中由于离心而受到强烈的影响。为了最大限度地减少这种影响，成功开发了一种新的方案。使用传统的试剂和设备，它需要一个多星期，但细胞压缩减少到没有或最低的变形可能。
In cell culture, cell structures suffer strong impact due to centrifugation during processing for electron microscope observation. In order to minimise this effect, a new protocol was successfully developed. Using conventional reagents and equipments, it took over one week, but cell compression was reduced to none or the lowest deformation possible.