Identification and characterization of multiple curcumin synthases from the herb Curcuma longa
Identification and characterization of multiple curcumin synthases from the herb Curcuma longa
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DOI：
10.1016/j.febslet.2009.07.029
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发表时间：
2009-09-03
期刊：
影响因子：
3.5
通讯作者：
Horinouchi, Sueharu
中科院分区：
文献类型：
--
作者：
Katsuyama, Yohei;Kita, Tomoko;Horinouchi, Sueharu
姜黄素类化合物是从草本植物姜黄（Curcuma longa）中分离的重要的药学化合物。另外两个III型聚酮酶，命名为CURS2和CURS3，能够姜黄素类化合物的合成进行了鉴定和表征。体外分析显示，CURS2优选阿魏酰辅酶A作为起始底物，CURS3优选阿魏酰辅酶A和对香豆酰辅酶A。这些结果表明，CURS2合成姜黄素或脱甲氧基姜黄素，CURS3合成姜黄素，双脱甲氧基姜黄素和脱甲氧基姜黄素。底物的可用性和三种不同的酶的表达水平能够姜黄素类化合物的合成与不同的底物特异性可能会影响姜黄和不同品种的姜黄素类化合物的组合物。(C)2009年欧洲生物化学学会联合会。由Elsevier B出版。V.保留所有权利。
Curcuminoids are pharmaceutically important compounds isolated from the herb Curcuma longa. Two additional type III polyketide synthases, named CURS2 and CURS3, that are capable of curcuminoid synthesis were identified and characterized. In vitro analysis revealed that CURS2 preferred feruloyl-CoA as a starter substrate and CURS3 preferred both feruloyl-CoA and p-coumaroyl-CoA. These results suggested that CURS2 synthesizes curcumin or demethoxycurcumin and CURS3 synthesizes curcumin, bisdemethoxycurcumin and demethoxycurcumin. The availability of the substrates and the expression levels of the three different enzymes capable of curcuminoid synthesis with different substrate specificities might influence the composition of curcuminoids in the turmeric and in different cultivars. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.