Patterning expression of regenerative growth factors using high intensity focused ultrasound.
Patterning expression of regenerative growth factors using high intensity focused ultrasound.
复制标题
使用高强度聚焦超声对再生生长因子进行模式化表达。
DOI：
10.1089/ten.tec.2013.0518
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Franceschi,RennyT
中科院分区：
文献类型：
--
作者：
Wilson,ChristopherG;Martín-Saavedra,FranciscoM;Padilla,Frédéric;Fabiilli,MarioL;Zhang,Man;Baez,AlexanderM;Bonkowski,ChristopherJ;Kripfgans,OliverD;Voellmy,Richard;Vilaboa,Nuria;Fowlkes,JBrian;Franceschi,RennyT
生长因子梯度的时间和空间控制对于组织图案化和分化是至关重要的。在伤口愈合和组织再生期间，组织再生也需要重新启动该发育程序。设计重建生长因子梯度的方法仍然是再生医学的核心挑战。在目前的研究中，我们开发了一种新的基因治疗方法，用于骨再生中两种重要的生长因子血管内皮生长因子和骨形态发生蛋白2的时空控制，该方法涉及将高强度聚焦超声应用于经热激活和配体诱导的基因开关工程改造的细胞。转基因表达的诱导被紧密地定位在细胞支架构建体内至约30mm3的亚体积，并且转基因表达的幅度和投影面积通过超声暴露的强度和持续时间来调节。超声激活转基因表达的条件导致最小的细胞毒性和支架损伤。生长因子表达的局部区域还建立了信号传导活性的梯度，这表明该方法产生的生长因子表达模式将在组织发育和再生的基础和应用研究中发挥作用。
Temporal and spatial control of growth factor gradients is critical for tissue patterning and differentiation. Reinitiation of this developmental program is also required for regeneration of tissues during wound healing and tissue regeneration. Devising methods for reconstituting growth factor gradients remains a central challenge in regenerative medicine. In the current study we develop a novel gene therapy approach for temporal and spatial control of two important growth factors in bone regeneration, vascular endothelial growth factor, and bone morphogenetic protein 2, which involves application of high intensity focused ultrasound to cells engineered with a heat-activated- and ligand-inducible gene switch. Induction of transgene expression was tightly localized within cell-scaffold constructs to subvolumes of ∼30 mm3, and the amplitude and projected area of transgene expression was tuned by the intensity and duration of ultrasound exposure. Conditions for ultrasound-activated transgene expression resulted in minimal cytotoxicity and scaffold damage. Localized regions of growth factor expression also established gradients in signaling activity, suggesting that patterns of growth factor expression generated by this method will have utility in basic and applied studies on tissue development and regeneration.
登录
查看更多内容
影响因子：
4.1
作者：
Patel, Zarana S.;Young, Simon;Tabata, Yasuhiko;Jansen, John A.;Wong, Mark E. K.;Mikos, Antonios G.
通讯作者：
Mikos, Antonios G.
影响因子：
12.4
作者：
Yazici, Cemal;Takahata, Masahiko;Schwarz, Edward M.
通讯作者：
Schwarz, Edward M.
DOI：
10.1121/1.426854
发表时间：
1999-04
期刊：
The Journal of the Acoustical Society of America
影响因子：
--
作者：
W. F. Walker
通讯作者：
W. F. Walker
DOI：
10.2106/00004623-199907000-00002
发表时间：
1999-07-01
影响因子：
5.3
作者：
Lieberman, JR;Daluiski, A;Witte, ON
通讯作者：
Witte, ON
影响因子：
--
作者：
Rutherford, RB;Moalli, M;Krebsbach, PH
通讯作者：
Krebsbach, PH