Thermal Shock and Ciprofloxacin Act Orthogonally on Pseudomonas aeruginosa Biofilms.
Thermal Shock and Ciprofloxacin Act Orthogonally on Pseudomonas aeruginosa Biofilms.
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热休克和环丙沙星对铜绿假单胞菌生物膜的影响
DOI：
10.3390/antibiotics10081017
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发表时间：
2021-08-21
期刊：
影响因子：
--
通讯作者：
Nuxoll E
中科院分区：
文献类型：
--
作者：
Aljaafari H;Gu Y;Chicchelly H;Nuxoll E
细菌生物膜感染是医疗植入物的主要责任，由于它们对抗生素和宿主免疫反应的抗性。热冲击可以杀死已形成的生物膜，一些证据表明抗生素可能会增强这种功效，尽管其本身的效果不足。然而，这种相互作用的性质尚不清楚，这使得将热冲击整合到植入物感染治疗中的努力变得复杂。本研究旨在确定这些处理是真正协同还是简单正交（即，独立）。铜绿假单胞菌生物膜的不同的架构和固定相人口密度进行各种热冲击，抗生素暴露，或其组合，并检查后立即处理或随后的再孵育。组合处理的群体减少与单独处理的减少的乘积相匹配，表明其正交性。然而，再孵育显示出二元行为，其中具有超过临界因子（~104）的立即种群减少的生物膜在再孵育期间完全死亡，而具有较小的立即减少的生物膜重新生长。这一关键因素与初始种群密度和实现立即减少的处理组合无关。虽然抗生素似乎没有直接增强热冲击，但它们对实现生物膜消除的临界种群减少的贡献可以使治疗表现出强烈的协同作用，从而大大降低所需的热冲击强度。
Bacterial biofilm infections are a major liability of medical implants, due to their resistance to both antibiotics and host immune response. Thermal shock can kill established biofilms, and some evidence suggests antibiotics may enhance this efficacy, despite having an insufficient effect themselves. The nature of this interaction is unclear, however, complicating efforts to integrate thermal shock into implant infection treatment. This study aimed to determine whether these treatments were truly synergistic or simply orthogonal (i.e., independent). Pseudomonas aeruginosa biofilms of different architectures and stationary-phase population density were subjected to various thermal shocks, antibiotic exposures, or combinations thereof, and examined either immediately after treatment or after subsequent reincubation. Population decreases from the combination treatment matched the product of the decreases of individual treatments, indicating their orthogonality. However, reincubation showed binary behavior, where biofilms with an immediate population decrease beyond a critical factor (~104) died off completely during reincubation, while biofilms with a smaller immediate decrease regrew. This critical factor was independent of the initial population density and the combination of treatments that achieved the immediate decrease. While antibiotics do not appear to enhance thermal shock directly, their contribution to achieving a critical population decrease for biofilm elimination can make the treatments appear strongly synergistic, strongly decreasing the intensity of thermal shock needed.
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影响因子：
4.6
作者：
Cooper, H. J.;Della Valle, C. J.
通讯作者：
Della Valle, C. J.
影响因子：
3.1
作者：
Pijls, Bart G.;Sanders, Ingrid M. J. G.;Nelissen, Rob G. H. H.
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Nelissen, Rob G. H. H.
影响因子：
28.2
作者：
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通讯作者：
Myers, Jenny
影响因子：
4.9
作者：
Hajdu, Stefan;Holinka, Johannes;Presterl, Elisabeth
通讯作者：
Presterl, Elisabeth
DOI：
10.1016/j.msec.2015.11.036
发表时间：
2016-03-01
影响因子：
7.9
作者：
Perez-Diaz, M.;Alvarado-Gomez, E.;Martinez-Gutierrez, F.
通讯作者：
Martinez-Gutierrez, F.