EMERGENCE OF MULTIPOTENT HEMATOPOIETIC-CELLS IN THE YOLK-SAC AND PARAAORTIC SPLANCHNOPLEURA IN MOUSE EMBRYOS, BEGINNING AT 8.5 DAYS POSTCOITUS
EMERGENCE OF MULTIPOTENT HEMATOPOIETIC-CELLS IN THE YOLK-SAC AND PARAAORTIC SPLANCHNOPLEURA IN MOUSE EMBRYOS, BEGINNING AT 8.5 DAYS POSTCOITUS
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DOI：
10.1073/pnas.92.3.773
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发表时间：
1995-01-31
影响因子：
11.1
通讯作者：
CUMANO, A
中科院分区：
文献类型：
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作者：
GODIN, I;DIETERLENLIEVRE, F;CUMANO, A
我们通过体外方法表明，在10-25体节阶段（妊娠8.5-9.5天）的小鼠胚胎体内可以检测到多能造血细胞-即，在肝定殖之前（28-32对体节）。有趣的是，造血细胞平行出现在这个位置，腹主动脉旁的内脏胸膜，并在卵黄囊，在那里他们代表了新一代的原始造血干细胞。从卵黄囊和腹主动脉旁内脏胸膜细胞制备物中获得了可分化为成熟B细胞的类卵黄细胞克隆，但未从胚胎体的其他组织中获得。这些B细胞前体首先检测到10体节的阶段左右，此后，他们最初的分钟数增加平行卵黄囊和腹主动脉旁的内脏，这表明他们的出现在这两个网站是同时的。通过单细胞操作，我们表明，这些前体细胞可以产生B和T淋巴细胞和骨髓细胞，这些前体细胞可以被定义为多能造血细胞。
We show by an in vitro approach that multipotent hemopoietic cells can be detected in the body of the mouse embryo between the stages of 10-25 somites (8.5-9.5 days of gestation)-i.e., prior to liver colonization (28-32 pairs of somites). Interestingly, hemopoietic cells appear in parallel in this location, the paraaortic splanchnopleura, and in the yolk sac, where they represent a new generation by reference to the primitive hemopoietic stem cells. Lymphoid cell clones, which could differentiate into mature B cells, were obtained from yolk sac and paraaortic splanchnopleura cell preparations but not from other tissues of the embryonic body. These B-cell precursors were first detected around the stage of 10 somites; thereafter, their initial minute numbers increased in parallel in the yolk sac and the paraaortic splanchnopleura, suggesting that their emergence in the two sites was simultaneous. By single cell manipulation, we show that these precursors can generate B and T lymphocytes and myeloid cells; these precursors can thus be defined as multipotent hemopoietic cells.