A phosphorylated transcription factor regulates sterol biosynthesis in Fusarium graminearum
A phosphorylated transcription factor regulates sterol biosynthesis in Fusarium graminearum
复制标题
磷酸化转录因子调节禾谷镰刀菌中甾醇的生物合成
DOI：
10.1038/s41467-019-09145-6
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发表时间：
2019-03-15
影响因子：
16.6
通讯作者：
Yin, Yanni
中科院分区：
文献类型：
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作者：
Liu, Zunyong;Jian, Yunqing;Yin, Yanni
在许多真核生物中，固醇的生物合成是由转录因子SREBP控制的。在这里，我们发现SREBP同源基因不参与调节禾谷镰刀菌中的甾醇生物合成，禾谷镰刀菌是一种世界性的谷物真菌。相反，在这种生物体中，固醇的产生受另一种转录因子FgSR的控制，该转录因子形成一个同源二聚体，并与其目标基因启动子的一个16个碱基的顺式元件结合，其中包含两个保守的CGAA重复序列。FgSR被MAP激酶FgHog1磷酸化，磷酸化的FgSR与染色质重塑复合体SWI/SNF在靶基因上相互作用，导致转录增强。有趣的是，FgSR同源基因只存在于丝状真菌和乳链霉菌中。此外，FgSR主要通过调节脱氧雪腐镰刀菌烯醇的生物合成和对植物抗毒素的反应来控制毒力。
Sterol biosynthesis is controlled by transcription factor SREBP in many eukaryotes. Here, we show that SREBP orthologs are not involved in the regulation of sterol biosynthesis in Fusarium graminearum, a fungal pathogen of cereal crops worldwide. Instead, sterol production is controlled in this organism by a different transcription factor, FgSR, that forms a homodimer and binds to a 16-bp cis-element of its target gene promoters containing two conserved CGAA repeat sequences. FgSR is phosphorylated by the MAP kinase FgHog1, and the phosphorylated FgSR interacts with the chromatin remodeling complex SWI/SNF at the target genes, leading to enhanced transcription. Interestingly, FgSR orthologs exist only in Sordariomycetes and Leotiomycetes fungi. Additionally, FgSR controls virulence mainly via modulating deoxynivalenol biosynthesis and responses to phytoalexin.