Effects of glycolic acid on desquamation-regulating proteinases in human stratum corneum
Effects of glycolic acid on desquamation-regulating proteinases in human stratum corneum
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DOI：
10.1111/j.0906-6705.2005.00224.x
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发表时间：
2005-01-01
影响因子：
3.6
通讯作者：
Funasaka, Y
中科院分区：
文献类型：
--
作者：
Horikoshi, T;Matsumoto, M;Funasaka, Y
为了研究乙醇酸 (GA) 在人角质层中的功能机制，我们在人体皮肤上局部使用 GA（50% w/v，pH 0.9）30 分钟后，监测组织蛋白酶 D 样（CD）和糜蛋白酶样（SCCE）蛋白酶的变化 3 周。早期，第2天观察到下层角质层凝聚力减弱，从30分钟到第2天，角质层上层活性CD量显着减少，而下层则保持正常。相反，活性 SCCE 的量在实验期间没有变化。角质层表面pH值在30分钟时急剧下降至pH 2，并在治疗后1天略微恢复至pH 3左右。从第9天到第19天，观察到角质细胞面积减少，上层活性CD数量显着长期增加。在一项体外研究中，由于 CD 在其最佳 pH 值下被激活，脱屑调节蛋白酶的活性在 pH 3 左右显着增加。这些结果表明，GA 通过至少两种不同的机制发挥作用，通过 pH 3 左右的酸化来急性激活下层中的 CD，同时使上层中的 CD 失活，以及在 GA 处理后几周内从头开始 CD 产生的长期增强。
In order to investigate the mechanism of glycolic acid (GA) function in human stratum corneum, we monitored changes in cathepsin D-like (CD) and chymotrypsin-like (SCCE) proteinases for 3 weeks following topical GA application (50% w/v, pH 0.9) for 30 min to human skin. In the early phase, weakened stratum corneum cohesion in the lower layers was observed on day 2 and the amount of active CD in the upper layer of the stratum corneum was significantly decreased from 30 min until day 2, whereas that in the lower layer remained normal. In contrast, the amount of active SCCE showed no change during the experimental period. The surface pH of the stratum corneum drastically decreased to pH 2 at 30 min and slightly recovered to around pH 3 until 1 day after treatment. From 9 to 19 days, a decrease in corneocyte cell area and a remarkable long-term increase in the amount of active CD in the upper layer were observed. In an in vitro study, the activities of desquamation-regulating proteinases were shown to have remarkably increased at around pH 3, due to activation of CD at its optimal pH. These results suggest that GA functions via at least two different mechanisms, acute activation of CD in the lower layer by acidification around pH 3, along with inactivation of CD in the upper layer, and long-term enhancement of de novo CD production in the few weeks following GA treatment.