Inhibition by spermine of the inner membrane permeability transition of isolated rat heart mitochondria
Inhibition by spermine of the inner membrane permeability transition of isolated rat heart mitochondria
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DOI：
10.1016/0014-5793(92)81217-a
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发表时间：
1992-11
期刊：
影响因子：
3.5
通讯作者：
R. Lapidus;P. M. Sokolove
中科院分区：
文献类型：
--
作者：
R. Lapidus;P. M. Sokolove
评价了精胺对离体大鼠心脏线粒体内膜渗透性转变的影响。使用一系列试剂（叔丁基过氧化氢、苯胂氧化物、羧甲基纤维素和升高的Ca2+和无机磷酸盐浓度）触发渗透性转变，并通过Ca2+释放、线粒体肿胀和吡啶核苷酸氧化进行监测。根据这三个标准，精胺抑制了这种转变。测定抑制的C50为0.38 ± 0.06（SD）mM。
The effect of spermine on the permeability transition of the inner mitochondrial membrane of isolated rat heart mitochondria was evaluated. The permeability transition was triggered using a series of agents (t‐butyl hydroperoxide, phenylarsine oxide, carboxyatractylate, and elevated Ca2+and inorganic phosphate concentrations), and was monitored via Ca2+‐release, mitochondrial swelling and pyridine nucleotide oxidation. By all three criteria, spermine inhibited the transition. A C50of 0.38 ± 0.06 (SD) mM was measured for inhibition.