Chloroplastic protein NRIP1 mediates innate immune receptor recognition of a viral effector
Chloroplastic protein NRIP1 mediates innate immune receptor recognition of a viral effector
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DOI：
10.1016/j.cell.2007.12.031
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发表时间：
2008-02-08
期刊：
影响因子：
64.5
通讯作者：
Dinesh-Kumar, S. P.
中科院分区：
文献类型：
--
作者：
Caplan, Jeffrey L.;Mamillapalli, Padmavathi;Dinesh-Kumar, S. P.
植物先天免疫依赖于富含亮氨酸重复序列（NB-LRR）免疫受体家族对病原体效应分子的识别。以前的研究表明，TIR-NB-LRR家族成员N免疫受体通过其TIR结构域间接识别烟草花叶病毒（TMV）的50 kDa解旋酶（p50）结构域。我们已经鉴定出一种N受体相互作用蛋白NRIP 1，它直接与N的TIR结构域和p50相互作用。NRIP 1是一种功能性的硫转移酶，是N提供对TMV的完全抗性所必需的。有趣的是，通常定位于叶绿体的NRIP 1被p50效应子募集到细胞质和细胞核。因此，NRIP 1仅在p50效应物存在下与N相互作用。我们的研究结果表明，叶绿体蛋白密切参与病原体识别。我们建议，N的激活需要一个包含p50效应器和NRIP 1的预识别复合物。
Plant innate immunity relies on the recognition of pathogen effector molecules by nucleotide-binding-leucine-rich repeat (NB-LRR) immune receptor families. Previously we have shown the N immune receptor, a member of TIR-NB-LRR family, indirectly recognizes the 50 kDa helicase (p50) domain of Tobacco mosaic virus (TMV) through its TIR domain. We have identified an N receptor-interacting protein, NRIP1, that directly interacts with both N's TIR domain and p50. NRIP1 is a functional rhodanese sulfur-transferase and is required for N to provide complete resistance to TMV. Interestingly, NRIP1 that normally localizes to the chloroplasts is recruited to the cytoplasm and nucleus by the p50 effector. As a consequence, NRIP1 interacts with N only in the presence of the p50 effector. Our findings show that a chloroplastic protein is intimately involved in pathogen recognition. We propose that N's activation requires a prerecognition complex containing the p50 effector and NRIP1.