A NOVEL RECOVERY-SYSTEM FOR CULTURED-CELLS USING PLASMA-TREATED POLYSTYRENE DISHES GRAFTED WITH POLY(N-ISOPROPYLACRYLAMIDE)
A NOVEL RECOVERY-SYSTEM FOR CULTURED-CELLS USING PLASMA-TREATED POLYSTYRENE DISHES GRAFTED WITH POLY(N-ISOPROPYLACRYLAMIDE)
复制标题
DOI：
10.1002/jbm.820271005
复制
发表时间：
1993-10-01
期刊：
影响因子：
--
通讯作者：
SAKURAI, Y
中科院分区：
文献类型：
--
作者：
OKANO, T;YAMADA, N;SAKURAI, Y
聚（N-异丙基丙烯酰胺）（PIPAAm）在低于 32 摄氏度时表现出完全展开的链构象，并在高温下表现出塌陷的紧凑构象。这种独特的温度响应聚合物被接枝到商业聚苯乙烯皿的表面上，并用作温度开关，用于创建低于 32 摄氏度的亲水表面和高于 32 摄氏度的疏水表面。在 37 摄氏度下，牛内皮细胞和大鼠肝细胞在 PIPAAm 移植表面上的细胞附着和生长表现出与商业化培养皿相似的行为。只需将温度降低到聚合物转变温度以下（塌陷），就可以观察到两种细胞类型都从 PIPAAm 接枝表面分离。通过这种方法回收的细胞保持了与原代培养细胞中发现的几乎相同的底物粘附性、生长和分泌活性，这与胰蛋白酶处理损伤的培养细胞中发现的功能受损相反。这些结果提供了强有力的证据，表明 PIPAAm 接枝表面作为热开关对于逆转细胞附着和分离非常有效，而不会损坏细胞。通过这种技术，细胞培养表面的特性可以很容易地可逆地转化为 PIPAAm 接枝聚合物的亲水性和疏水性涂层。 (C) 1993 约翰威利父子公司
Poly(N-isopropyl acrylamide) (PIPAAm) demonstrated a fully expanded chain conformation below 32-degrees-C and a collapsed, compact conformation at high temperatures. This unique temperature responsive polymer was grafted onto surfaces of commercial polystyrene dishes and used as temperature switches for creating hydrophilic surfaces below 32-degrees-C and hydrophobic surfaces above 32-degrees-C. Cell attachment and the growth of bovine endothelial cells and rat hepatocytes on PIPAAm-grafted surfaces at 37-degrees-C demonstrated similar behavior to the commercialized culture dishes. Both cell types were observed to detach from the PIPAAm-grafted surface simply by reducing the temperature below the polymer transition temperature (collapse). Cells recovered by this method maintained substrate adhesivity, growth, and secretion activities nearly identical to those found in primary cultured cells in contrast to the compromised function found in cultured cells damaged by trypsinization. These results provide strong evidence that PIPAAm-grafted surfaces, as thermal switches are very effective for reversing cell attachment and detachment without cell damage. Properties of cell culture surfaces can be readily transformed by this technique reversibly into hydrophilic and hydrophobic coatings of PIPAAm-grafted polymers. (C) 1993 John Wiley & Sons, Inc.