Chromatin docking and exchange activity enhancement of RCC1 by histones H2A and H2B
Chromatin docking and exchange activity enhancement of RCC1 by histones H2A and H2B
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DOI：
10.1126/science.292.5521.1540
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发表时间：
2001-05-25
期刊：
影响因子：
56.9
通讯作者：
Macara, IG
中科院分区：
文献类型：
--
作者：
Nemergut, ME;Mizzen, CA;Macara, IG
Ran鸟苷三磷酸酶（GTdR）控制核质转运、有丝分裂纺锤体形成和核膜组装。这些功能依赖于RAN特异性交换因子RCC 1（染色体凝集调节因子1）与染色质的结合。我们发现，RCC 1直接结合到单核小体和组蛋白H2 A和H2 B。RCC 1利用这些组蛋白结合爪蟾精子染色质，RCC 1与核小体或组蛋白的结合刺激RCC 1的催化活性。我们认为RCC 1与H2 A/H2 B的对接建立了Ran-CTP梯度的极性，驱动核膜组装、核运输和其他核事件。
The Ran guanosine triphosphatase (GTPase) controls nucleocytoplasmic transport, mitotic spindle formation, and nuclear envelope assembly. These functions rely on the association of the Ran-specific exchange factor, RCC1 (regulator of chromosome condensation 1), with chromatin. We find that RCC1 binds directly to mononucleosomes and to histones H2A and H2B. RCC1 utilizes these histones to bind Xenopus sperm chromatin, and the binding of RCC1 to nucleosomes or histones stimulates the catalytic activity-of RCC1, We propose that the docking of RCC1 to H2A/H2B establishes the polarity of the Ran-CTP gradient that drives nuclear envelope assembly, nuclear transport, and other nuclear events.