Dynamic expression of corticotropin-releasing hormone and urocortin in estrogen induced-cholestasis pregnant rat
Dynamic expression of corticotropin-releasing hormone and urocortin in estrogen induced-cholestasis pregnant rat
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DOI：
10.1016/j.reprotox.2016.07.019
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发表时间：
2016-10-01
影响因子：
3.3
通讯作者：
Wang, Xiaodong
中科院分区：
文献类型：
--
作者：
Zhou, Fan;Gao, Bingxin;Wang, Xiaodong
妊娠期肝内胆汁淤积症（ICP）并发急性胎盘-胎儿缺氧。促肾上腺皮质激素释放激素（CRH）和尿皮质素（UCN）是胎盘血流的血管舒张调节剂。用炔雌醇（EE）复制大鼠胆汁淤积模型，测定孕14-21天（DG）血清及胎盘CRH/UCN。对照组大鼠母体血清或胎盘CRH/UCN水平在14- 21 DG期间相对一致。与对照组大鼠相比，EE治疗组大鼠在21 DG时血清CRH降低。关于血清UCN，我们观察到与对照组相比，EE治疗组大鼠在17 DG时降低，在21 DG时升高。此外，我们观察到一个显着的减少胎盘CRH/UCN的表达在17或19 DG EE治疗的大鼠与对照组相比。血清胆汁酸水平与胎盘CRH/UCN表达呈负相关。EE诱导的胆汁淤积大鼠可作为进一步研究ICP中CRH/UCN失调的病理机制的良好模型。(C)2016 Elsevier Inc. All rights reserved.
Intrahepatic cholestasis of pregnancy(ICP) is complicated by acute placental-fetal hypoxia. Corticotropin-releasing hormone(CRH) and urocortin(UCN) are vasodilatory regulators of blood flow in the placenta. An ethinylestradiol(EE)-induced cholestasis rat model was reproduced and serum/placental CRH/UCN were detected during 14-21 days of gestation(DG). Maternal serum or placental CRH/UCN levels in the control rats were relatively consistent during 14-21DG. Serum CRH was reduced in the EE-treated rats compared with the control rats at 21DG. Regarding serum UCN, we observed a decrease at 17DG as well as an increase at 21DG in the EE-treated rats compared with the controls. Moreover, we observed a noticeable reduction of placental CRH/UCN expression at 17 or 19DG in the EE-treated rats compared with the control rats. The serum bile acids levels exhibited an inverse correlation with placental CRH/UCN expression. EE-induced cholestasis rats might serve as a good model to further investigate the pathological mechanism underlying CRH/UCN dysregulation in ICP. (C) 2016 Elsevier Inc. All rights reserved.