CHAOPTIN, A CELL-SURFACE GLYCOPROTEIN REQUIRED FOR DROSOPHILA PHOTORECEPTOR CELL MORPHOGENESIS, CONTAINS A REPEAT MOTIF FOUND IN YEAST AND HUMAN
CHAOPTIN, A CELL-SURFACE GLYCOPROTEIN REQUIRED FOR DROSOPHILA PHOTORECEPTOR CELL MORPHOGENESIS, CONTAINS A REPEAT MOTIF FOUND IN YEAST AND HUMAN
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DOI：
10.1016/0092-8674(88)90518-1
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发表时间：
1988-01-29
期刊：
影响因子：
64.5
通讯作者：
ZIPURSKY, SL
中科院分区：
文献类型：
--
作者：
REINKE, R;KRANTZ, DE;ZIPURSKY, SL
Chaoptin是一种感光细胞特异性膜蛋白。对chaoptin突变体的分析表明，这种糖蛋白在感光细胞形态发生中起着关键作用。我们从cDNA序列中推断了chaoptin的一级结构，并研究了其膜拓扑结构。Chaoptin主要由41个潜在的两亲性重复组成。在酵母和人类中已经报道了显著的同源重复序列，表明它们作为结构和/或功能基序的普遍重要性。体外合成的Chaoptin与粗面内质网微粒体膜以完整的方式结合，与其体内提取特征一致。体外合成和加工的chaoptin的蛋白水解消化的抗性表明，它主要定位于脂双层的细胞外小叶。这些数据与chaoptin参与感光细胞膜之间粘附的提议一致。
Chaoptin is a photoreceptor cell-specific membrane protein. Analysis of chaoptin mutants demonstrates that this glycoprotein plays a critical role in photoreceptor cell morphogenesis. We have deduced chaoptin''s primary structure from the cDNA sequence and examined its membrane topology. Chaoptin is largely composed of 41 potentially amphipathic repeats. Remarkably homologous repeats have been reported in both yeast and human, suggesting their general importance as a structural and/or functional motif. Chaoptin synthesized in vitro is associated with rough endoplasmic reticulum microsomal membrane in an integral fashion, consistent with its extraction characteristics in vivo. The resistance of proteolytic digestion of in vitro synthesized and processed chaoptin suggests that it is primarily localized to the extracellular leaflet of the lipid bilayer. These data are consistent with the proposal that chaoptin is involved in the adhesion between photoreceptor cell membranes.