Effects of shear stress on wound-healing angiogenesis in the rabbit ear chamber.
Effects of shear stress on wound-healing angiogenesis in the rabbit ear chamber.
复制标题
剪切应力对兔耳室伤口愈合血管生成的影响。
DOI：
--
复制
发表时间：
1997
影响因子：
2.2
通讯作者：
Akira Kamiya
中科院分区：
文献类型：
--
作者：
Shigeru Ichioka;M. Shibata;K. Kosaki;Yuko Sato;Kiyonori Harii;Akira Kamiya
近年来的研究表明，管壁剪切应力在血管系统的生理适应中起着重要的作用。本研究通过研究组织修复过程中体内微血管的形态和血流动力学变化，探讨持续壁剪切应力对创面愈合血管生成的影响。兔在耳室植入后第0天至第23天口服α 1受体阻滞剂吡唑嗪（水中50 mg/L），以增加外周血流量。从第7天到第23天，用活体视频显微镜记录腔内的微血管。利用计算机图像分析系统，定量测定了血管化组织覆盖的腔室相对面积（%）、长入速率（mm2/天）、总血管面积（mm2）和小静脉（直径在20-40微米）的壁剪切应力水平（dyne/cm2）。第7 ~ 19天，哌唑嗪处理动物的相对面积显著增加。治疗组的腔室比对照组更早被血管化组织完全覆盖。治疗组最终总血管面积增大21%。治疗组剪切应力的时间过程表现为初始升高（比对照组增加1.44倍），然后逐渐向对照组水平降低。这些发现表明，伤口愈合血管生成可能部分参与了微血管对剪切应力的适应性反应。
Recent studies indicate that wall shear stress plays a significant role in the physiological adaptation of the vascular system. This study focused on the effect of sustained wall shear stress on wound-healing angiogenesis by exploring the morphologic and hemodynamic changes in developing microvessels in vivo through the tissue repair process. Rabbits were treated with the alpha 1 blocker prazosin (50 mg/L in water) orally from Day 0 to Day 23 after implantation of ear chambers to increase peripheral blood flow. The microvasculature in the chamber was recorded from Day 7 to Day 23 by using an intravital videomicroscope. The relative area of the chamber covered by vascularized tissue (%), the rate of ingrowth (mm2/day), the total vascular area (mm2), and the wall shear stress level (dyne/cm2) in venules (diameter in 20-40 microns) were quantified using a computerized image analysis system. The relative area increased significantly in the prazosin-treated animals from Days 7 to 19. The chamber of the treated group was completely covered with vascularized tissue earlier than that of the control group. The final total vascular area was larger by 21% in the treated group. The time course of shear stress in the treated group showed an initial elevation (1.44 times increase vs the control) followed by a gradual decrease toward the control level. These findings suggest that wound-healing angiogenesis may be partly involved in the adaptive response of microvasculature to shear stress.
正常生长期间的微血管发育和血流量减少：引入新模型。
DOI：
10.1152/ajpheart.1991.260.6.h1966
发表时间：
1991
期刊：
The American journal of physiology
影响因子：
--
作者：
Wang,DH;Prewitt,RL
通讯作者：
Prewitt,RL
影响因子：
2.2
作者：
ROESEL, JF;NANNEY, LB
通讯作者：
NANNEY, LB