An efficient one-pot four-segment condensation method for protein chemical synthesis.
An efficient one-pot four-segment condensation method for protein chemical synthesis.
复制标题
DOI：
10.1002/anie.201500051
复制
发表时间：
2015-05
影响因子：
--
通讯作者：
Shan Tang;Yanyan Si;Zhi-Peng Wang;Kunrong Mei;Xin Chen;Jing Cheng;Ji‐Shen Zheng;Lei Liu
中科院分区：
文献类型：
--
作者：
Shan Tang;Yanyan Si;Zhi-Peng Wang;Kunrong Mei;Xin Chen;Jing Cheng;Ji‐Shen Zheng;Lei Liu
使用一锅法的连续肽连接可以提高蛋白质化学合成的效率。虽然一锅法三段连接已经得到广泛应用，但一锅法四段连接的稳健方法迄今为止尚未开发。在此，我们报告了一种新的一锅多片段肽连接方法，可用于缩合多达四个片段，操作简单，效率高。它的实用性通过一锅四段合成植物蛋白，crambin和人类趋化因子hCCL21来证明。
Successive peptide ligation using a one-pot method can improve the efficiency of protein chemical synthesis. Although one-pot three-segment ligation has enjoyed widespread application, a robust method for one-pot four-segment ligation had to date remained undeveloped. Herein we report a new one-pot multisegment peptide ligation method that can be used to condense up to four segments with operational simplicity and high efficiency. Its practicality is demonstrated by the one-pot four-segment synthesis of a plant protein, crambin, and a human chemokine, hCCL21.