Mapping and haplotyping of the flanking region of the polyembryony locus in Citrus unshiu Marcow
Mapping and haplotyping of the flanking region of the polyembryony locus in Citrus unshiu Marcow
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DOI：
10.2503/jjshs1.77.109
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发表时间：
2008-04-01
影响因子：
--
通讯作者：
Omura, Mitsuo
中科院分区：
文献类型：
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作者：
Nakano, Michiharu;Shimizu, Tokurou;Omura, Mitsuo
多胚现象是柑桔属植物中广泛存在的一种由珠心组织中的体细胞胚胎发生而产生的一种特殊的真种子繁殖系统。为了进一步完善柑橘多胚性遗传图谱，我们利用“宫川”（Citrus unshiu Marcow）ד特罗维塔”（Trovita）橙子（Citrus unshiu Marcow）的F_1群体进行了群体分离分析，获得了多胚性遗传标记。sinensis（L.）Osbeck））x 'Miyagawa wase'.利用EST序列设计的1681对引物和12个碱基随机引物进行组合分离分析，并对引物进行了改进，系统地筛选了标记。获得了与多胚性基因座连锁的5个标记，其中2个标记转化为序列特征扩增区（SCAR）标记。这些标记被整合到之前由EST衍生的切割扩增多态性序列（CAPS）标记构建的框架图中，并放置在之前报道的K-9连锁群上。新标记的精细连锁群覆盖了多胚性位点两侧的47 cM。从后代及其亲本的多胚位点周围区域的单倍型分析，它表明'Kiyomi'在多胚位点附近的侧翼区域具有重组单倍型。该连锁图和单倍型结构的信息有助于分子标记辅助选择和柑橘多胚性位点的进一步研究。
Polyembryony, which is a specific true-seed propagation system generated by somatic embryogenesis in nucellar tissue, occurs widely in the genus Citrus. To refine the genetic map of the polyembryony locus in Citrus, we obtained DNA markers from bulked segregation analysis using an F, population derived from 'Kiyomi' ('Miyagawa wase' (Citrus unshiu Marcow) x 'Trovita' orange (C. sinensis (L.) Osbeck)) x 'Miyagawa wase'. A total of 1681 primers designed from expressed sequence tag (EST) sequences and random 12-base primers were utilized for bulked segregation analysis with modification to screen markers systematically. Five markers linked to the polyembryony locus were obtained, of which two of these markers were converted to sequence characterized amplified region (SCAR) markers. These markers were integrated to a framework map previously constructed by EST-derived cleaved amplified polymorphic sequence (CAPS) markers and placed on the previously reported K-9 linkage group. The refined linkage group with new markers covered 47 cM flanking the polyembryony locus. From haplotype analysis of the region around the polyembyony locus in the progenies and their parents, it was shown that 'Kiyomi' had a recombinant haplotype in the flanking region close to the polyembryony locus. Information concerning the linkage map and haplotype structure contributes to marker-assisted selection and further study of the polyembryony locus in Citrus.