Normal growth and differentiation in a spontaneously immortalized near-diploid human keratinocyte cell line, NIKS
Normal growth and differentiation in a spontaneously immortalized near-diploid human keratinocyte cell line, NIKS
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DOI：
10.1046/j.1523-1747.2000.00869.x
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发表时间：
2000-03-01
影响因子：
6.5
通讯作者：
O'Connor, SL
中科院分区：
文献类型：
--
作者：
Allen-Hoffmann, BL;Schlosser, SJ;O'Connor, SL
我们报告了一个自发永生化的人角质形成细胞系NIKS的分离和鉴定。该细胞系在无胸腺裸鼠中不具有致瘤性，并保持体外生长的细胞类型特异性要求。NIKS细胞表达的转化生长因子-α、转化生长因子-β 1、表皮生长因子受体、c-myc和角蛋白14 mRNA的稳态水平与亲本BC-1-Ep角质形成细胞株相当。BC-1-Ep和NIKS角质形成细胞对基质附着丧失的反应产生相似水平的角质层包膜和核小体片段化。DNA指纹分析结果证实NIKS细胞来源于亲本BC-1-Ep角质形成细胞。由于8号染色体长臂的额外等染色体，NIKS细胞含有47条染色体，并且近二倍体核型似乎在重复传代中稳定。在器官型培养中，NIKS角质形成细胞形成完全复层的鳞状上皮。父母和永生化的角质形成细胞的超微结构分析显示丰富的桥粒，半桥粒，和生产的基底层。我们对NIKS细胞的研究结果支持了自发永生化与鳞状分化或凋亡能力的改变无关的观察结果。NIKS人角质形成细胞系是研究复层鳞状上皮生长和分化的重要新工具。
We report the isolation and characterization of a spontaneously immortalized human keratinocyte cell line, NIKS. The cell line is not tumorigenic in athymic nude mice and maintains cell-type-specific requirements for growth in vitro. NIKS cells express steady-state levels of transforming growth factor-alpha, transforming growth factor-beta 1, epidermal growth factor receptor, c-myc, and keratin 14 mRNAs comparable with the parental BC-1-Ep keratinocyte strain. BC-1-Ep and NIKS keratinocytes produce similar levels of cornified envelopes and nucleosomal fragmentation in response to loss of substrata attachment. DNA fingerprinting results confirm that the NIKS cells originated from the parental BC-1-Ep keratinocytes. NIKS cells contain 47 chromosomes due to an extra isochromosome of the long arm of chromosome 8, and the near-diploid karyotype appears to be stable with repeated passage. A fully stratified squamous epithelium is formed by the NIKS keratinocytes in organotypic culture. Ultrastructural analysis of both the parental and immortalized keratinocytes reveals abundant desmosomes, hemidesmosomes, and the production of a basal lamina. Our findings with the NIKS cells support the observation that spontaneous immortalization is not linked to alterations in squamous differentiation or the ability to undergo apoptosis. The NIKS human keratinocyte cell line is an important new tool for the study of growth and differentiation in stratified squamous epithelia.