Systematic screening and identification of antigens recognized by monoclonal antibodies raised against the developing lateral olfactory tract
Systematic screening and identification of antigens recognized by monoclonal antibodies raised against the developing lateral olfactory tract
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DOI：
10.1002/neu.20105
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发表时间：
2005-02-15
期刊：
影响因子：
--
通讯作者：
Hirata, T
中科院分区：
文献类型：
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作者：
Kawasaki, T;Takagi, Y;Hirata, T
在发育过程中，嗅球轴突在由无数分子组成的复杂微环境中导航，构建一个称为侧嗅束的束。轴突本身也表达数千种不同的分子。在本研究中，我们生产并表征了六种单克隆抗体，它们以独特的模式标记侧嗅束及其周围环境。标记谱表明，每种抗体识别的抗原分子在发育中的侧嗅束周围异质分布。我们开发了一种有效的筛选方法，通过结合 COS-7 细胞中 cDNA 文库的表达和随后的细胞免疫组织化学染色来鉴定抗原分子。系统筛选成功地鉴定了所有单克隆抗体的特异性 cDNA 克隆，这些克隆很可能编码抗原分子，因此揭示了小鼠正在发育的侧嗅道的局部成分的分子性质。 (C) 2004 年 Wiley 期刊。公司
During development, olfactory bulb axons navigate a complex microenvironment composed of myriad molecules to construct a bundle called the lateral olfactory tract. The axons themselves also express thousands of different molecules. In the present study, we produced and characterized six monoclonal antibodies that label the lateral olfactory tract and its surroundings in a unique pattern. The labeling profiles suggested that the antigen molecules recognized by each antibody are heterogeneously distributed around the developing lateral olfactory tract. We developed an efficient screening method to identify the antigen molecules by combining expression of a cDNA library in COS-7 cells and the subsequent immunohistochemical staining of the cells. The systematic screening successfully identified specific cDNA clones for all of the monoclonal antibodies, which highly probably coded for the antigen molecules, and therefore unveiled the molecular nature of local components that embrace the developing lateral olfactory tract in mice. (C) 2004 Wiley Periodicals. Inc.