Analysis of epidermal growth factor receptor dimerization by BS³ cross-linking.
Analysis of epidermal growth factor receptor dimerization by BS³ cross-linking.
复制标题
通过 BS 交联分析表皮生长因子受体二聚化。
DOI：
10.1007/978-1-4939-1789-1_3
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Chapkin,RobertS
中科院分区：
文献类型：
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作者：
Turk,HarmonyF;Chapkin,RobertS
Dimerization of receptor tyrosine kinases is a well-characterized process. It is imperative for the activation of many receptors, including the epidermal growth factor receptor (EGFR). EGFR has been shown to be regulated by a number of factors, including lipid raft localization. For example, alteration of the lipid raft localization of EGFR has been demonstrated to modify receptor dimerization. This protocol describes an assay to quantify EGFR dimers using BS3cross-linking. BS3cross-linking is well suited for this purpose because of its length, water solubility, and membrane impermeability. Although this protocol is written specifically for EGFR, the assay can be extrapolated in order to characterize dimerization of other receptor tyrosine kinases.
