Quantitative Metabolomic Profiling of Serum and Urine in DSS-Induced Ulcerative Colitis of Mice by 1H NMR Spectroscopy
Quantitative Metabolomic Profiling of Serum and Urine in DSS-Induced Ulcerative Colitis of Mice by 1H NMR Spectroscopy
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DOI：
10.1021/pr100547y
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发表时间：
2010-12-01
影响因子：
4.4
通讯作者：
Storr, Martin
中科院分区：
文献类型：
--
作者：
Schicho, Rudolf;Nazyrova, Alsu;Storr, Martin
大量代谢产物的定量分析是检测溃疡性结肠炎(UC)等炎症性肠病(IBD)生物标志物的一种很有前途的方法。我们用葡聚糖硫酸钠(DSS)诱导小鼠实验性UC，并利用H-1核磁共振波谱和定量(靶向)分析表征了53种血清和69种尿代谢物，以区分疾病和健康动物。在DSS结肠炎期间，尿液中肌酸、肉碱和甲胺增加，尿液中酮体、次黄嘌呤和色氨酸抗氧化性代谢物增加，而血清中酮体、次黄嘌呤和色氨酸抗氧化性代谢物增加，而在血清中，葡萄糖和Krebs循环中间产物显著减少，从而区分健康和DSS治疗组小鼠实验性结肠炎。
Quantitative profiling of a large number of metabolic compounds is a promising method to detect biomarkers in inflammatory bowel diseases (IBD) such as ulcerative colitis (UC) We induced an experimental form of UC in mice by treatment with dextran sulfate sodium (DSS) and characterized 53 serum and 69 urine metabolites by use of H-1 NMR spectroscopy and quantitative ("targeted ) analysis to distinguish between diseased and healthy animals Hierarchical multivariate orthogonal partial least-squares (OPLS) models were developed to detect and predict separation of control and DSS treated mice DSS treatment resulted in weight loss, colonic inflammation, and increase in myeloperoxidase activity Metabolomic patterns generated from the OPLS data clearly separated DSS-treated from control mice with a slightly higher predictive power (Q(2)) for serum (0 73) than urine (0 71) During DSS colitis creatine, carnitine, and methylamines increased in urine while in serum, maximal increases were observed for ketone bodies, hypoxanthine, and tryptophan Antioxidant metabolites decreased in urine whereas in serum, glucose and Krebs cycle intermediates decreased strongly Quantitative metabolic profiling of serum and urine thus discriminates between healthy and DSS-treated mice Analysis of serum or urine seems to be equally powerful for detecting experimental colitis, and a combined analysis offers only a minor improvement