R-spondin3 is required for mouse placental development
R-spondin3 is required for mouse placental development
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DOI：
10.1016/j.ydbio.2006.08.018
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发表时间：
2007-01-01
影响因子：
2.7
通讯作者：
Okamoto, Hitoshi
中科院分区：
文献类型：
--
作者：
Aoki, Motoko;Mieda, Michihiro;Okamoto, Hitoshi
小鼠R-spondin 3（Rspo 3）是R-spondin蛋白家族的成员，其特征在于富含弗林蛋白酶样半胱氨酸的结构域和tbrombospondin 1型重复。Rspo 3被认为是一种分泌性分子，促进Wnt/β-连环蛋白信号通路。我们产生的小鼠携带突变Rspo 3等位基因，其中lacZ编码区取代第一外显子的编码区。纯合子突变小鼠在胚胎第10天左右死亡，原因是胎盘的谷胱甘肽层形成受损。Rspo 3在迷路的尿囊成分中表达。在纯合子突变胎盘中，胎儿血管没有渗透到绒毛膜中，并且在绒毛膜滋养层细胞中编码转录因子胶质细胞缺失-1（Gcm 1）的Gcm 1的表达显著降低。这些发现表明，Rspo 3在绒毛膜和尿囊膜之间的相互作用中起着至关重要的作用。(c)2006年爱思唯尔公司All rights reserved.
Mouse R-spondin3 (Rspo3) is a member of the R-spondin protein family, which is characterized by furin-like cysteine-rich domains and a tbrombospondin type 1 repeat. Rspo3 has been proposed to function as a secretory molecule that promotes the Wnt/beta-catenin signaling pathway. We generated mice bearing a mutant Rspo3 allele in which a lacZ-coding region replaced the coding region of the first exon. The homozygous mutant mice died at about embryonic day 10, due to impaired formation of the labyrinthine layer of the placenta. Rspo3 was expressed in the allantoic component of the labyrinth. In the homozygous mutant placentas, fetal blood vessels did not penetrate into the chorion, and expression of Gcm1, encoding the transcription factor glial cells missing-1 (Gcm1), was dramatically reduced in the chorionic trophoblast cells. These findings suggest a critical role for Rspo3 in the interaction between chorion and allantois in labyrinthine development. (c) 2006 Elsevier Inc. All rights reserved.