Region-specific expression of immunoregulatory proteins on microglia in the healthy CNS
Region-specific expression of immunoregulatory proteins on microglia in the healthy CNS
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DOI：
10.1002/glia.20663
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发表时间：
2008-06-01
期刊：
影响因子：
6.2
通讯作者：
Biber, Knut
中科院分区：
文献类型：
--
作者：
De Haas, Alexander H.;Boddeke, Hendrikus W. G. M.;Biber, Knut
根据中枢神经系统（CNS）的高度空间组织性，大多数CNS疾病显示出区域性分布。虽然小胶质细胞已被确定为各种CNS疾病的关键参与者，但尚不清楚小胶质细胞是否显示区域特异性特性。因此，本研究旨在评估健康成年小鼠CNS不同区域中不同小胶质细胞表型的存在。使用离体流式细胞术分析，分析了CD 11b、CD 40、CD 45、CD 80、CD 86、F4/80、TREM-2b、MHCII、CXCR 3、CCR 9和CCR 7的表面表达。这些免疫调节标志物中的大多数在小胶质细胞上发现，并且在表达水平上显示出显著的区域特异性差异。这些发现相当程度上证实了在健康的、未受攻击的成年小鼠中枢神经系统中小胶质细胞之间存在免疫多样性。(C)2008 Wiley-Liss，Inc.
In accordance with a high degree of spatial organization in the central nervous system (CNS), most CNS diseases display a regional distribution. Although microglia have been established as key players in various CNS diseases, it is not yet clear whether microglia display region-specific properties. Therefore, this study aimed to evaluate the existence of distinct microglia phenotypes in various regions of the healthy, adult mouse CNS. Using ex vivo flow cytometric analysis surface expression of CD11b, CD40, CD45, CD80, CD86, F4/80, TREM-2b, MHCII, CXCR3, CCR9, and CCR7 were analyzed. Most of these immunoregulatory markers were found on microglia and showed significant region-specific differences in expression levels. These findings considerably corroborate the existence of immunological diversity among microglia in the healthy, unchallenged CNS of adult mice. (C) 2008 Wiley-Liss, Inc.