Comparative proteomic study of liver lipid droplets and mitochondria in mice housed at different temperatures
Comparative proteomic study of liver lipid droplets and mitochondria in mice housed at different temperatures
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不同温度下小鼠肝脏脂滴和线粒体的比较蛋白质组学研究
DOI：
10.1002/1873-3468.13509
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发表时间：
2019-07-12
期刊：
影响因子：
3.5
通讯作者：
Zhang, Shuyan
中科院分区：
文献类型：
--
作者：
Liu, Qingfeng;Zhou, Ziyun;Zhang, Shuyan
将实验室小鼠置于约23 °C的环境中，使其处于慢性冷应激下。在热中性、标准和低温下饲养的小鼠肝脏中的代谢变化仍然未知。在本研究中，我们分离脂滴和线粒体从他们的肝脏中的比较蛋白质组学研究，旨在调查的变化。蛋白质组学分析显示，低温条件下，线粒体三羧酸循环（TCA循环）和视黄醇代谢增强，氧化磷酸化作用不明显，提示肝脏线粒体可能通过增加生物合成途径中TCA循环能力，以及视黄醇代谢，帮助肝脏适应。基于蛋白质组学和免疫印迹结果，周脂蛋白5和主要尿蛋白显著增加，而线粒体丙酮酸载体在寒冷条件下显著减少，表明它们参与肝脏适应。
Laboratory mice are standardly housed at around 23 °C, setting them under chronic cold stress. Metabolic changes in the liver in mice housed at thermoneutral, standard and cold temperatures remain unknown. In the present study, we isolated lipid droplets and mitochondria from their livers in a comparative proteomic study aiming to investigate the changes. According to proteomic analysis, mitochondrial tricarboxylic acid cycle (TCA cycle) and retinol metabolism are enhanced, whereas oxidative phosphorylation is not affected obviously under cold conditions, suggesting that liver mitochondria may increase TCA cycle capacity in biosynthetic pathways, as well as retinol metabolism, to help the liver to adapt. Based on proteomic and immunoblotting results, perilipin 5 and major urinary proteins are increased significantly, whereas mitochondrial pyruvate carrier is decreased dramatically under cold conditions, indicating their involvement in liver adaptation.