Changes in visual fields and lateral geniculate nucleus in monkey laser-induced high intraocular pressure model
Changes in visual fields and lateral geniculate nucleus in monkey laser-induced high intraocular pressure model
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DOI：
10.1016/j.exer.2008.02.004
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发表时间：
2008-05-01
影响因子：
3.4
通讯作者：
Hara, Hideaki
中科院分区：
文献类型：
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作者：
Sasaoka, Masaaki;Nakamura, Katsuki;Hara, Hideaki
猴眼可用于眼科疾病的眼科研究，因为它们的组织学和功能特性与人类非常相似。激光诱导猴高眼压模型是眼科研究，特别是青光眼研究的常用模型。尽管使用该模型的几项研究集中在视野、视网膜神经节细胞（RGC）和外侧膝状体核（LGN）的变化上，但尚未在同一只猴子中建立这些变化之间的明确关系。因此，我们研究视野的变化，RGC和LGN的数量，和胶质纤维酸性蛋白（GFAP）的免疫组织化学在LGN中的两只猴子。视野敏感度、RGC数目和LGN神经元密度均降低。RGC的丧失与视野敏感度下降之间的关系取决于与中央凹的偏心率。此外，LGN免疫组织化学显示，在接受来自高IOP眼的神经元输入的层中，GFAP表达的增加大于接受来自对侧未治疗眼的神经元输入的层中。从这些结果中，我们认为，青光眼可能会导致神经胶质功能的变化，不仅在视网膜，但也在视觉通路，这种中枢神经系统的变化可能是一个标志性的神经病变青光眼，在其他神经退行性疾病。(c)2008爱思唯尔有限公司保留所有权利。
Monkey eyes are useful for ophthalmologic research into eye diseases because their histological and functional properties are very similar to those of humans. The monkey laser-induced high intraocular pressure (IOP) model is a common model for ophthalmologic research, especially into glaucoma. Although several studies using this model have focused on changes in visual field, retinal ganglion cells (RGC), and lateral geniculate nucleus (LGN), clear relationships among these changes in one and the same monkey have not been established. We therefore examined visual field changes, RGC and LGN numbers, and glial fibrous acidic protein (GFAP) immunohistochemistry in the LGN in each of two monkeys. Visual field sensitivity, RGC number, and neuronal density of LGN were all decreased by high IOP. The relationship between loss of RGC and decrease in visual field sensitivity depended on the eccentricity from the fovea. Moreover, LGN immunohistochemistry revealed greater increases in GFAP expression in the layers receiving a neuronal input from the high IOP eye than in those receiving a neuronal input from the contralateral untreated eye. From these results, we suggest that glaucoma may lead to changes in glial function not only in the retina, but also in the visual pathway, and that such central nervous system changes may be a hallmark of neuropathy in glaucoma, as in other neurodegenerative diseases. (c) 2008 Elsevier Ltd. All rights reserved.