A decrease of cell proliferation by hypothermia in the hippocampus of the neonatal rat
A decrease of cell proliferation by hypothermia in the hippocampus of the neonatal rat
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DOI：
10.1016/j.brainres.2006.06.112
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发表时间：
2006-09-21
期刊：
影响因子：
2.9
通讯作者：
Murata, Yuji
中科院分区：
文献类型：
--
作者：
Kanagawa, Takeshi;Fukuda, Hiromitsu;Murata, Yuji
亚低温不仅对成人而且对新生儿的脑缺氧缺血性损伤都有潜在的治疗作用。然而，低温对发育中的大脑的副作用仍然不清楚，大脑中发生了大量的神经发生。我们研究了神经前体细胞的增殖，全身应用胸苷类似物5-溴脱氧尿苷（BrdU）在新生大鼠在严重的低温环境。将大鼠幼崽分为两组，低温组（30 ℃：n=10）和常温组（37 ℃：n=10）。在每种环境中放置21 h后，腹腔注射100 mg/kg/day的BrdU标记分裂细胞，然后在24 h处死。我们检查了脑室周围的脑室下区和齿状回的颗粒下区的BrdU标记细胞的数量。在低温环境中，BrdU标记细胞的数量显着减少，在齿状回，但不是在脑室周围区。因此，严重的低温环境导致新生大鼠神经发生减少。这些观察结果是值得注意的临床低温治疗脑缺氧缺血性损伤在围产期。(c)2006 Elsevier B. V.保留所有权利。
Hypothermia is a potential therapy for cerebral hypoxic ischemic injury of not only adults but also neonates. However, the side effects of hypothermia in the developing brain, where a massive amount of neurogenesis occurs, remain unclear. We investigated the proliferation of neural progenitor cells by systemic application of the thymidine analog 5-bromodeoxyuridine (BrdU) in neonatal rats in a severe hypothermic environment. The rat pups were divided into two groups, a hypothermia group (30 degrees C: n=10) and a normothermia group (37 degrees C: n=10). After the pups were placed for 21 h in each environment, 100 mg/kg/day of BrdU was injected intraperitoneally to label dividing cells, and then the pups were sacrificed at 24 h. We examined the number of BrdU-labeled cells in the subventricular zone of the periventricle and the subgranular zone of the dentate gyrus. In the hypothermic environment, BrdU-labeled cells significantly decreased in number in the dentate gyrus, but not in the periventricular region. Thus, the severe hypothermic environment induced a decrease of neurogenesis in the neonatal rat. These observations are noteworthy regarding clinical hypothermia therapy following cerebral hypoxic ischemic injury during the perinatal period. (c) 2006 Elsevier B.V. All rights reserved.