Human Arf tumor suppressor specifically interacts with chromatin containing the promoter of rRNA genes
Human Arf tumor suppressor specifically interacts with chromatin containing the promoter of rRNA genes
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DOI：
10.1038/sj.onc.1207968
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发表时间：
2004-10-21
期刊：
影响因子：
8
通讯作者：
Seite, P
中科院分区：
文献类型：
--
作者：
Ayrault, O;Andrique, L;Seite, P
肿瘤抑制蛋白Arf(Alternative Read Frame)蛋白(人的p14(ARF)和小鼠的p19(ARF))主要定位于核仁中，与其亚细胞定位一致，已被证明与5.8S rRNA和B23/核磷蛋白特异地相互作用，并调节核糖体的生物发生。在这里，我们证明了p14(ARF)蛋白与染色质相互作用，并通过染色质免疫沉淀(CHIP)在包含rRNA基因启动子DNA序列的部分中被回收。此外，已被证明与p14(ARF)相互作用的拓扑异构酶I(Topo I)与含有染色质的p14(ARF)共沉淀。考虑到Topo I在rRNA转录中的功能，这些数据与p14(Arf)-Topo I复合体在rRNA转录和/或成熟中的作用是一致的。
The tumor suppressor Arf (Alternative Reading Frame) protein (p14(ARF) in human and p19(ARF) in mouse) is mainly located in the nucleolus consistent with its subcellular localization, the protein has been shown to specifically interact with 5.8S rRNA and with B23/Nucleophosmin and to regulate ribosome biogenesis. Here, we show that the p14(ARF) protein interacts with chromatin and is recovered by chromatin immunoprecipitation (ChIP) in a fraction that contains a DNA sequence of the rRNA gene promoter. In addition, topoisomerase I (Topo I) that has been shown to interact with p14(ARF) coprecipitates with p14(ARF) containing chromatin. These data, in view of the function for Topo I in rRNA transcription, are consistent with a role for the p14(ARF)-Topo I complex in rRNA transcription and/or maturation.