Extra small virus-like particles (XSV) and nodavirus associated with whitish muscle disease in the giant freshwater prawn, Macrobrachium rosenbergii
Extra small virus-like particles (XSV) and nodavirus associated with whitish muscle disease in the giant freshwater prawn, Macrobrachium rosenbergii
复制标题
DOI：
10.1046/j.1365-2761.2003.00486.x
复制
发表时间：
2003-09-01
影响因子：
2.5
通讯作者：
Bonami, JR
中科院分区：
文献类型：
--
作者：
Qian, D;Shi, Z;Bonami, JR
最近在浙江、江苏、上海、广西和广东等省记录了中国养殖的罗氏沼虾的一种疾病。这种疾病发生在幼虫后(PL)，其临床症状是肌肉出现白色，尤其是在腹部。在一些孵化场，死亡率可能达到100%。对发病的PL匀浆进行阴性染色后的透射电子显微镜观察表明，存在两种类型的病毒颗粒：一种是无包膜的二十面体形状，直径为26-27 nm；另一种是直径小得多的病毒颗粒，直径约14-16 nm，称为超小病毒颗粒(XSV)。这种大病毒的基因组有两个单链RNA片段(RNA-1和RNA-2)，分别为3kb和1.2kb。杂交试验证实，这一大型病毒与从法属西印度群岛一家孵化场的病虾中分离到的罗森伯吉诺达病毒(M.Rosenbergii nodaVirus，Mr NV)密切相关。它非常小的体积和假想的生化和生物学特征表明XSV是一种新的甲壳类病毒。由于XSV一直被发现与较大的病毒(NodaVirus)有关，并位于患病动物的肌肉和连接细胞中，因此它可能是一种自主病毒、帮助型病毒或卫星样病毒。
A disease of Macrobrachium rosenbergii, the giant freshwater prawn, farmed in China was recently recorded in Zhejiang, Jiangsu, Shanghai, Guangxi and Guangdong provinces. The clinical sign of the disease, which develops in post-larvae (PL), is a whitish appearance of the muscles, particularly noticeable in the abdomen. Mortalities may reach 100% in some hatcheries. Investigations by transmission electron microscopy after negative staining of diseased PL homogenates showed the presence of two types of viral particles: one, unenveloped, icosahedral in shape, 26 - 27 nm in diameter, the second, much smaller, about 14 - 16 nm in diameter, designated extra small virus particle (XSV). The large virus has a genome with two pieces of ssRNA (RNA-1 and RNA-2), of 3 and 1.2 kb, respectively. Hybridization tests confirmed that this large virus is closely related to M. rosenbergii nodavirus (MrNV) which was isolated from diseased prawns in a hatchery in the French West Indies. Its very small size and hypothesized biochemical and biological characteristics suggest XSV is a new type of crustacean virus. As XSV has always been found associated with the larger virus ( nodavirus) and is located in muscle and connective cells of diseased animals, it could be an autonomous virus, a helper-type virus or a satellite-like virus.