A phosphoinositide-specific phospholipase C pathway elicits stress-induced Ca2+ signals and confers salt tolerance to rice
A phosphoinositide-specific phospholipase C pathway elicits stress-induced Ca2+ signals and confers salt tolerance to rice
复制标题
磷酸肌醇特异性磷脂酶 C 途径引发应激诱导的 Ca2 信号并赋予水稻耐盐性
DOI：
10.1111/nph.14426
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发表时间：
2017-05-01
期刊：
影响因子：
9.4
通讯作者：
Zhang, Wenhua
中科院分区：
文献类型：
--
作者：
Li, Li;Wang, Fawei;Zhang, Wenhua
在动物细胞中，磷脂酶C（PLC）主要水解磷脂酰肌醇-4，5-二磷酸[PtdIns（4，5）P-2]，生成第二信使甘油二酯（DAG）和肌醇1，4，5-三磷酸[Ins（1，4，5）P-3]，调节多种生物学过程。相比之下，植物PLC信号转导的分子机制和生理意义仍有待充分阐明。本研究鉴定了一个水稻（Oryza sativa cv）磷脂酰肌醇-4-磷酸（PtdIns 4P）水解酶，并诱导胁迫诱导的钙离子信号调节水稻耐盐性。在地上部中，PtdIns（4，5）P-2的表达量是PtdIns（4，5）P-2的28倍。OsPLC 1不仅能在体内外将PtdIns（4，5）P2转化为DAG和Ins（1，4，5）P-3，而且能更有效地将PtdIns 4P转化为DAG和Ins（1，4，5）P-3。OsPLC 1介导的胁迫诱导的Ca 2+信号转导是控制叶片Na+积累、建立植物耐盐性的重要途径，本研究确定了水稻PtdIns 4P库的转化途径和生理功能，揭示了盐胁迫反应中磷酸肌醇与PLC介导的Ca 2+信号转导之间的联系。
In animal cells, phospholipase C (PLC) isoforms predominantly hydrolyze phosphatidylinositol- 4,5-biphosphates [PtdIns(4,5) P-2] into the second messengers diacylglycerol (DAG) and inositol 1,4,5-trisphosphate [Ins(1,4,5) P-3] to regulate diverse biological processes. By contrast, the molecular mechanisms and physiological significance of PLC signaling in plants still awaits full elucidation. Here, we identified a rice (Oryza sativa cv) PI-PLC, OsPLC1, which preferred to hydrolyze phosphatidylinositol-4-phosphate (PtdIns4P) and elicited stress-induced Ca2+ signals regulating salt tolerance.Analysis by ion chromatography revealed that the concentration of PtdIns4P was c. 28 times of that of PtdIns(4,5) P-2 in shoots. OsPLC1 not only converted PtdIns(4,5) P2 but also and even more efficiently - converted PtdIns4P into DAG and Ins(1,4,5) P-3 in vitro and in vivo.Salt stress induced the recruitment of OsPLC1 from cytoplasm to plasma membrane, where it hydrolyzed PtdIns4P. The stress-induced Ca2+ signaling was dependent on OsPLC1, and the PLC-mediated Ca2+ signaling was essential for controlling Na+ accumulation in leaf blades, thus establishing whole plant salt tolerance.Our work identifies a conversion pathway and physiological function for PtdIns4P pools in rice and reveals the connection between phosphoinositides and Ca2+ signals mediated by PLC during salt stress responses.