Pyrrolysine Analogs as Substrates for Bacterial Pyrrolysyl-tRNA Synthetase in Vitro and in Vivo
Pyrrolysine Analogs as Substrates for Bacterial Pyrrolysyl-tRNA Synthetase in Vitro and in Vivo
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DOI：
10.1271/bbb.110653
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发表时间：
2012-01-01
影响因子：
1.6
通讯作者：
Hojo, Hironobu
中科院分区：
文献类型：
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作者：
Katayama, Hidekazu;Nozawa, Kayo;Hojo, Hironobu
吡咯赖氨酸-tRNA(Pyl) 复合物由吡咯赖氨酰-tRNA 合成酶 (PylRS) 产生。在本研究中，我们研究了 Desulfitobacter hafnience PylRS 的底物特异性。 PylRS 在体外将各种 L-赖氨酸衍生物掺入 tRNA(Pyl) 中。此外，PylRS/tRNA(Pyl)对通过大肠杆菌表达系统将这些赖氨酸衍生物引入重组蛋白中，表明该PylRS/tRNA(Pyl)对可用于蛋白质工程技术。
Pyrrolysine-tRNA(Pyl) complex is produced by pyrrolysyl-tRNA synthetase (PylRS). In this study, we investigated the substrate specificity of Desulfitobacterium hafnience PylRS. PylRS incorporated various L-lysine derivatives into tRNA(Pyl) in vitro. In addition, the PylRS/tRNA(Pyl) pair introduced these lysine derivatives into the recombinant protein by the Escherichia coli expression system, indicating that this PylRS/tRNA(Pyl) pair can be used in protein engineering technology.