Mitochondrial oxidant generation and oxidative damage in ames dwarf and GH transgenic mice
Mitochondrial oxidant generation and oxidative damage in ames dwarf and GH transgenic mice
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DOI：
10.1007/s11357-001-0012-6
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发表时间：
2001-07-01
期刊：
影响因子：
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通讯作者：
Romanick, M
中科院分区：
文献类型：
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作者：
Brown-Borg, H;Johnson, WT;Romanick, M
衰老与蛋白质、脂质和DNA氧化损伤的积累有关。旨在防止氧化损伤的细胞机制随着年龄的增长和与衰老相关的疾病而减弱。一种长寿的小鼠，Ames侏儒，表现出生长激素缺乏和抗氧化防御能力增强。相比之下，过度表达生长激素的动物抗氧化能力受到抑制，寿命只有野生型小鼠的一半。在这项研究中，我们检测了Ames侏儒和野生型小鼠肝脏线粒体产生H-202，并测定了这些动物不同组织中蛋白质、脂质和DNA的氧化损伤水平。在琥珀酸存在下，矮小肝脏线粒体(24个月)产生的H_2O_2少于正常肝脏(p
Aging is associated with an accumulation of oxidative damage to proteins, lipids and DNA. Cellular mechanisms designed to prevent oxidative damage decline with aging and in diseases associated with aging. A long-lived mouse, the Ames dwarf, exhibits growth hormone deficiency and heightened antioxidative defenses. In contrast, animals that over express GH have suppressed antioxidative capacity and live half as long as wild type mice. In this study, we examined the generation of H 202 from liver mitochondria of Ames dwarf and wild type mice and determined the level of oxidative damage to proteins, lipids and DNA in various tissues of these animals. Dwarf liver mitochondria (24 months) produced less H2O2 than normal liver in the presence of succinate (p