Hemocytic immune responses triggered by CpG ODNs in shrimp Litopenaeus vannamei.
Hemocytic immune responses triggered by CpG ODNs in shrimp Litopenaeus vannamei.
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DOI：
10.1016/j.fsi.2012.09.016
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发表时间：
2013
影响因子：
4.7
通讯作者：
R. Sun;L. Qiu;Feng Yue;Lingling Wang;Rui Liu;Zhi Zhou;Huan Zhang;L. Song
中科院分区：
文献类型：
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作者：
R. Sun;L. Qiu;Feng Yue;Lingling Wang;Rui Liu;Zhi Zhou;Huan Zhang;L. Song
CpG寡核苷酸(CpG ODN)又称细菌DNA或人工合成的寡核苷酸，对多种病原体具有明显的免疫保护作用，被广泛应用于哺乳动物的功能免疫刺激剂或疫苗佐剂。本研究构建了富含CpG基因的真核表达载体pUC57-CpG，并用其刺激凡纳滨对虾，检测其对对虾血细胞总数、血细胞凋亡率、循环血细胞再生、吞噬能力和产生活性氧(ROS)的影响，以揭示CpG ODN可能的保护机制。注射pUC57-CpG后，刺激后第1天，小鼠外周血白细胞总数显著下降(P<0.01)，降至2.56×107cell/mL，细胞凋亡率增加(P<0.01)，是对照组的1.72倍。同时，循环血细胞的再生也有类似的变化趋势，刺激后第1天显著增加。吞噬百分率和吞噬指数等吞噬活性在整个实验期间呈上升趋势，刺激后第1天ROS水平较对照组增加22%(P<0.05)。以上结果提示，pUC57-CpG可促进循环血细胞的凋亡和再生，增强其吞噬功能和ROS的产生，从而增强机体对病原体感染的免疫力。
CpG oligodeoxynucleotides (CpG ODNs), also called bacterial DNA or synthetic oligodeoxynucleotides, can induce apparent immunity protection against various pathogens, and they are widely used as functional immunostimulant or vaccine adjuvant in mammals. In the present study, CpG-rich plasmid pUC57-CpG was constructed and employed to stimulate the shrimp Litopenaeus vannamei, and the total hemocyte count, percentage of apoptotic hemocytes, regeneration of circulating hemocytes, the ability of phagocytosis and generation of reactive oxygen species (ROS) were measured to reveal the possible protection mechanism of CpG ODNs. After the injection of pUC57-CpG, the total hemocyte count significantly decreased (p < 0.01) to 2.56 × 107cell/mL at the first day post stimulation, while the apoptosis increased (p < 0.01), which was 1.72-fold of that in control group. At the same time, the regeneration of circulating hemocytes fluctuated in a similar trend, and a significant increase was observed at the first day post stimulation. The phagocytotic activity including the percentage of phagocytosis and phagocytotic index, experienced an upward tend during the whole experimental period and the ROS level increased by 22% (p < 0.05) compared to that in the control group at first day post stimulation. These results together suggested that pUC57-CpG could promote the apoptosis and regeneration of circulating hemocytes, and enhance the phagocytosis and ROS production, which might contribute to the boosted immunity against the infection of pathogens.