Modulation of urinary siderophores by the diet, gut microbiota and inflammation in mice.
Modulation of urinary siderophores by the diet, gut microbiota and inflammation in mice.
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DOI：
10.1016/j.jnutbio.2016.11.014
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发表时间：
2017-03
期刊：
影响因子：
--
通讯作者：
Vijay-Kumar M
中科院分区：
文献类型：
--
作者：
Xiao X;Yeoh BS;Saha P;Tian Y;Singh V;Patterson AD;Vijay-Kumar M
哺乳动物的铁载体被认为在维持铁稳态方面起着关键作用。然而，哺乳动物铁载体的性质和功能还没有完全阐明。在本研究中，我们使用了一种成熟的细菌铁载体研究方法--铬天青S(CAS)法来检测和定量尿样中的哺乳动物铁载体。我们的研究表明，尿中的铁载体可以通过饮食、肠道微生物区系和炎症来改变。C57BL/6小鼠喂食含有大量植物化学物质的植物性食物，与喂食纯饲料的小鼠相比，尿中有更多的铁载体。在铁超载的情况下，尿铁载体上调，但不受其他被测试的营养状态的影响。此外，与常规小鼠相比，无菌小鼠的尿铁载体减少了50%，这表明微生物区系的生物转化在产生或刺激宿主新陈代谢以产生更多铁载体方面至关重要。炎症期间尿铁载体水平的改变表明宿主的健康状况影响全身的铁载体水平。这是第一份将尿铁载体作为一个整体进行测量的报告，描述了不同生理条件下铁载体水平是如何调节的。我们相信，我们的研究开辟了哺乳动物铁载体研究的新领域，我们以一种新颖的方式使用的技术具有应用于临床的潜力。
Mammalian siderophores are believed to play a critical role in maintaining iron homeostasis. However, the properties and functions of mammalian siderophores have not been fully clarified. In this study, we have employed Chrome Azurol S (CAS) assay which is a well-established method for bacterial siderophores study, to detect and quantify mammalian siderophores in urine samples. Our study demonstrates that siderophores in urine can be altered by diet, gut microbiota and inflammation. C57BL/6 mice, fed on plant-based chow diets which contain numerous phytochemicals, have more siderophores in the urine compared to those fed on purified diets. Urinary siderophores were upregulated in iron overload conditions, but not altered by other tested nutrients status. Further, germ-free mice displayed 50% reduced urinary siderophores, in comparison to conventional mice, indicating microbiota biotransformation is critical in generating or stimulating host metabolism to create more siderophores. Altered urinary siderophores levels during inflammation suggest that host health conditions influence systemic siderophores level. This is the first report to measure urinary siderophores as a whole, describing how siderophores levels are modulated under different physiological conditions. We believe that our study opens up a new field in mammalian siderophores research and the technique we used in a novel manner has the potential to be applied to clinical purpose.