Neurotrophins and their receptors in early axonal regeneration along muscle-vein-combined grafts
Neurotrophins and their receptors in early axonal regeneration along muscle-vein-combined grafts
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DOI：
10.1002/micr.10053
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发表时间：
2002-01-01
期刊：
影响因子：
2.1
通讯作者：
Battiston, B
中科院分区：
文献类型：
--
作者：
Pagnotta, A;Tos, P;Battiston, B
实验和临床研究表明，用骨骼肌填充的静脉段桥接周围神经缺损（肌-静脉联合移植物）可获得良好的神经修复效果。然而，沿着这种类型的移植物的神经纤维再生过程的分子基础仍然有待阐明。本研究的目的是验证两种神经营养因子，神经生长因子（NGF）和脑源性神经营养因子（BDNF），以及它们的受体，trkA和p75，在早期阶段的轴突再生的肌肉静脉联合移植物的表达。通过1厘米长的肌静脉联合移植物修复切断的大鼠坐骨神经，并在手术后立即（对照移植物）和手术后5天取出。移植物的纵向切片通过以下抗体进行免疫染色：抗-NGF、抗-BDNF、抗-trkA和抗p-75。抗胶质纤维酸性蛋白（抗GFAP）抗体用于识别雪旺细胞。结果显示，肌-静脉移植物内存在大量GFAP阳性的雪旺细胞。这些细胞中的许多对NGF、BDNF和p75反应，但不对trkA反应。在对照移植物中，手术后立即检测不到本研究中使用的任何抗体的免疫染色。这些观察结果表明，在手术后的早期，肌肉-静脉联合移植物为生长的轴突提供了一个特别有利于再生的环境，为我们提供了一个可能的解释，这种移植技术对周围神经修复的疗效。
Experimental and clinical studies have shown that a vein segment filled with skeletal muscle used to bridge a peripheral nerve defect (muscle-vein-combined graft) leads to good nerve repair. However, the molecular basis of the nerve fiber regeneration process along this type of graft still remains to be elucidated. The aim of this study was to verify the expression of two neurotrophins, nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF), as well as their receptors, trkA and p75, in an early stage of axonal regeneration in muscle-vein-combined grafts. Severed rat sciatic nerves were repaired by means of 1-cm-long musclevein-combined grafts and withdrawn immediately after surgery (control grafts) and 5 days after surgery. Longitudinal sections of grafts were immunostained by means of the following antibodies: anti-NGF, anti-BDNF, anti-trkA, and antip-75. An anti-glial fibrillar acid protein (anti-GFAP) antibody was used to recognize Schwann cells. Results showed the presence of a number of GFAP-positive Schwann cells inside the muscle-vein grafts. Many of these cells reacted for NGF, BDNF and p75, but not trkA. In control grafts, i.e., immediately after surgery, no immunostaining was detected for any of the antibodies used in this study. These observations suggest that, very early after surgery, the muscle-vein-combined graft offers to growing axons an environment particularly favorable for regeneration, providing us with a possible explanation for the efficacy of this grafting technique for peripheral nerve repair.