Detection of multiple respiratory pathogens during primary respiratory infection: nasal swab versus nasopharyngeal aspirate using real-time polymerase chain reaction
Detection of multiple respiratory pathogens during primary respiratory infection: nasal swab versus nasopharyngeal aspirate using real-time polymerase chain reaction
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DOI：
10.1007/s10096-009-0865-7
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发表时间：
2010-04-01
影响因子：
4.5
通讯作者：
Bont, L. J.
中科院分区：
文献类型：
--
作者：
Meerhoff, T. J.;Houben, M. L.;Bont, L. J.
在这项研究中，我们介绍了在原发性呼吸道疾病期间对婴儿呼吸道病毒的多重检测，研究了鼻拭子和鼻咽吸入物的敏感性，并评估了患者特征和病毒载量是否在敏感性中起作用。健康婴儿被包括在首次呼吸道感染的迹象中。收集成对的鼻咽抽吸液和鼻拭子。采用实时聚合酶链反应（Real-time polymerase chain reaction， PCR）对11种呼吸道病原菌进行检测。收集了98例婴儿的配对鼻咽吸痰和鼻拭子。鼻病毒（n = 67）和呼吸道合胞病毒（n = 39）检出最多。合并感染发生率为48% （n = 45）。鼻拭子对呼吸道合胞病毒（51%对100%）和鼻病毒（75%对97%）的敏感性低于鼻咽吸入液。鼻拭子的敏感性主要由周期阈值（CT）决定（p < 0.001）。在有严重症状的儿童中，拭子对呼吸道合胞病毒（而不是鼻病毒）的敏感性为100%（得分为千分之一日元11）。结论是，出于社区研究和监测目的，可以使用鼻拭子，尽管其敏感性低于抽吸，特别是用于检测呼吸道合胞病毒（RSV）感染的轻度病例。
In this study, we present the multiple detection of respiratory viruses in infants during primary respiratory illness, investigate the sensitivity of nasal swabs and nasopharyngeal aspirates, and assess whether patient characteristics and viral load played a role in the sensitivity. Healthy infants were included at signs of first respiratory tract infection. Paired nasopharyngeal aspirates and nasal swabs were collected. Real-time polymerase chain reaction (PCR) was carried out for 11 respiratory pathogens. Paired nasopharyngeal aspirates and nasal swabs were collected in 98 infants. Rhinovirus (n = 67) and respiratory syncytial virus (n = 39) were the most frequently detected. Co-infection occurred in 48% (n = 45) of the infants. The sensitivity of the nasal swab was lower than the nasopharyngeal aspirate, in particular, for respiratory syncytial virus (51% vs. 100%) and rhinovirus (75% vs. 97%). The sensitivity of the nasal swab was strongly determined by the cycle threshold (CT) value (p < 0.001). The sensitivity of the swab for respiratory syncytial virus, but not rhinovirus, was 100% in children with severe symptoms (score a parts per thousand yen11). It is concluded that, for community-based studies and surveillance purposes, the nasal swab can be used, though the sensitivity is lower than the aspirate, in particular, for the detection of mild cases of respiratory syncytial virus (RSV) infection.